Proceedings of the International Conference on Science and Education and Technology 2018 (ISET 2018)

Putative non-stereo specific dehalogenase from Antarctic of Psychotropic Bacillus sp IH01

Authors
Ismail Haruna, Hassana Abubakar, Roswanira Ab Wahab, Fahrul Huyop
Corresponding Author
Ismail Haruna
Available Online September 2018.
DOI
https://doi.org/10.2991/iset-18.2018.1How to use a DOI?
Keywords
Antarctic Bacterial Medium (ABM); 2,2-dichloropropionic acid (2,2-DCP); Dehalogenase; Biodegradation
Abstract

The main aim of the study was to isolate and identify any organism that can degrade 2,2-dichloropropionic acids (2,2DCP) from Antarctica soil and water environment. ntarctica is well known of its extreme cold temperature. In this study we successfully isolated at least six colonies (AA1, AA2, AB1, AB2, AC1 and AC2) from Antarctic environment of the soil sample grown on Antarctic Bacterial Medium. Of the six isolates, only isolate AA2 was could grow on a minimal media containing 2,2-dichloropropionic acids as a sole source of carbon. Analysis of the 16S rRNA gene sequence and biochemical tests, identified strain AA2 belongs to Bacillus sp.. The bacterium showed slow growth below 5oC, with cells doubling time of 32 hours. The chloride ion released was 0.657 mmolCl-/L on 20mM of 2, 2-DCP. In conclusion current study is important to explore biotechnological applications of cold adapted organisms and its special properties for fundamental applications. The new isolate can be used to improve the present bioremediation methods at low temperature. This is the first reported case about microbial growth on 2,2DCP (DALAPON) using a bacteria isolated from Antarctica.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the International Conference on Science and Education and Technology 2018 (ISET 2018)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
September 2018
ISBN
978-94-6252-648-8
ISSN
2352-5398
DOI
https://doi.org/10.2991/iset-18.2018.1How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Ismail Haruna
AU  - Hassana Abubakar
AU  - Roswanira Ab Wahab
AU  - Fahrul Huyop
PY  - 2018/09
DA  - 2018/09
TI  - Putative non-stereo specific dehalogenase from Antarctic of Psychotropic Bacillus sp IH01
BT  - Proceedings of the International Conference on Science and Education and Technology 2018 (ISET 2018)
PB  - Atlantis Press
SP  - 1
EP  - 4
SN  - 2352-5398
UR  - https://doi.org/10.2991/iset-18.2018.1
DO  - https://doi.org/10.2991/iset-18.2018.1
ID  - Haruna2018/09
ER  -