The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride
- https://doi.org/10.2991/absr.k.210810.030How to use a DOI?
- Incubation, Waste, Trichoderma viride
This study aims to determine the effect of substrate types on the crude activity of cellulase enzymes from local agricultural waste such as peanut shells, coconut fibers, brans, and teak leaves with the variations of incubation times (day 1, 3, 5, and 7) produced by Trichoderma viride. Enzyme activity was measured based on the amount of reducing sugar that produced by the DNS (Dinitrosalycilic Acid) method on a wavelength of 540 nm spectrophotometer. The results showed that the best substrate for cellulase production on coconut fibers substrate with an incubation period of 7 days which had the highest enzyme activity value of 1.340 U / ml. Coconut fibers contain the highest cellulose content compared to the other substrates. The lowest activity was shown by coconut fibers substrate at day one incubation time of 0.660 U / ml with the reducing sugar content of 0.594 mg/ml and protein content of 0.147 mg/ml. The complexity of the chemical composition of coconut fibers caused the longer time to degrade cellulose into glucose at day one than other three substrates.
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Pujiati AU - M W Ardhi AU - E Muktiani AU - N K Dewi AU - N Jadid AU - E N Prasetyo PY - 2021 DA - 2021/08/12 TI - The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride BT - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019) PB - Atlantis Press SP - 164 EP - 174 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210810.030 DO - https://doi.org/10.2991/absr.k.210810.030 ID - 2021 ER -