Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride

Authors
Pujiati, M W Ardhi, E Muktiani, N K Dewi, N Jadid, E N Prasetyo
Corresponding Author
Pujiati
Available Online 12 August 2021.
DOI
https://doi.org/10.2991/absr.k.210810.030How to use a DOI?
Keywords
Incubation, Waste, Trichoderma viride
Abstract

This study aims to determine the effect of substrate types on the crude activity of cellulase enzymes from local agricultural waste such as peanut shells, coconut fibers, brans, and teak leaves with the variations of incubation times (day 1, 3, 5, and 7) produced by Trichoderma viride. Enzyme activity was measured based on the amount of reducing sugar that produced by the DNS (Dinitrosalycilic Acid) method on a wavelength of 540 nm spectrophotometer. The results showed that the best substrate for cellulase production on coconut fibers substrate with an incubation period of 7 days which had the highest enzyme activity value of 1.340 U / ml. Coconut fibers contain the highest cellulose content compared to the other substrates. The lowest activity was shown by coconut fibers substrate at day one incubation time of 0.660 U / ml with the reducing sugar content of 0.594 mg/ml and protein content of 0.147 mg/ml. The complexity of the chemical composition of coconut fibers caused the longer time to degrade cellulose into glucose at day one than other three substrates.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
978-94-6239-417-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.210810.030How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Pujiati
AU  - M W Ardhi
AU  - E Muktiani
AU  - N K Dewi
AU  - N Jadid
AU  - E N Prasetyo
PY  - 2021
DA  - 2021/08/12
TI  - The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 164
EP  - 174
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.030
DO  - https://doi.org/10.2991/absr.k.210810.030
ID  - 2021
ER  -