Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
The Role of Synbiotic in Cobb-strain Broiler Performance Challenged with Campylobacter jejuni as a Substitute for Antibiotic Growth Promotor (AGP)
A E T H Wahyuni, T E M Nahak, M C C Malelak, V C Prakasita, S L Adrenalin
One of the feed additives that have long been used is Antibiotic Growth Promoter (AGP). But nowadays, the use of AGP as a growth promoter has been banned because it has negative impacts such as antimicrobial resistance. Campylobacter jejuni is a pathogenic bacterium that often infects birds, especially...
Antibacterial Effectiveness of Purple Yam (Dioscorea alata L) Sap Extract in Inhibiting the Growth of Staphylococcus aureus & Escherichia coli
A Nurfitriani, M Mahendradatta, A Laga, Zainal
Purple yam sap extracts have secondary metabolite components which can be utilized as an antibacterial property. Secondary metabolite compounds found from the result of the preliminary study include saponins, tannins, flavonoids, phenols, alkaloids, and triterpenoids. The aim of this study was to analyze...
Association of Chlamydia trachomatis, Mycoplasma spp., Ureaplasma urealyticum and U. parvum with Human Papillomavirus in Patients with Cervical Cancer
A L Sahara, F Ibrahim, M N Massi, A Yasmon
Besides human papillomavirus (HPV), the cervical carcinogenesis is also affected by many risk factors including pathogenic bacteria such as Chlamydia trachomatis (CT), Mycoplasma spp. (MS), Ureaplasma urealyticum (UU), and U. parvum (UP) infections. Thus, we studied the bacterial infections for 68 patients...
An Investigation of Spawn Growth of Pleurotus ostreatus in Heat-Tolerant Plastic Bags Using Rice and Corn as Substrates
S Afrida, K Willard, Lukman, Y Tamai
Indonesian mushroom farmers commonly produce Pleurotus ostreatus spawn in bottles. Bottles have several disadvantages: unsterilized lids, large weights, undetectable bacterial contaminations, and difficulties in removing the rest of mycelia around the edge of the bottle. Farmers rarely use plastic bags...
Antibacterial Effects of Crude Lectin Fraction Bioactive Compound of Red Macroalgae P. palmata and H. glandiforme from Southern Coast Java Island, Gunungkidul, Yogyakarta, Indonesia
C Anam, E Chasanah, B P Perdhana, ND Fajarningsih, N F Yusro, D Praseptiangga, A Yunus
Lectins or carbohydrate-binding proteins, are widely distributed in nature and also good candidates in such prospecting of algae. It may have been considered that binding specificity of lectins to some carbohydrates provokes to produce many unique biological activities, including cell agglutination,...
Gut-associated Lactic Acid Bacteria (LAB) in Apis nigrocincta (Smith)
C A Lombogia, M Tulung, J Posangi, T E Tallei
Honeybees are the world’s most important pollinator of food crops. Besides, they also produce honey, which is very beneficial for human health. Therefore the health of honeybees is essential to note. One aspect that contributes to the health of honeybees is the balance of the gut microbiota. Lactic acid...
Isolation and Identification of Bacteriocin-producing Bacillus Strain Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus)
C Hanim, V A Cahya, L M Yusiati, A Kurniawati
This research aimed to obtain Bacillus bacteria from the gastrointestinal tract (GIT) of native Indonesian chicken (Gallus domesticus) for bacteriocin production purposes against Escherichia coli. Bacillus bacteria were isolated from GIT (duodenum, jejunum, and ileum) on Trypticase Soya Agar (TSA+5%...
Optimization of Medium and Incubation Time in the Production of Antibacterial Compounds by Streptomyces sp. SA404
A Larasati, D Ryandini, O Oedjijono, D F Kusharyati
Streptomyces sp. SA404 was isolated from the mangrove rhizosphere in Segara Anakan Cilacap known for its production of antibacterial compounds. The production of antibacterial compounds might be influenced by nutrients (C and N sources) and incubation time. The aims of this study were to know the medium...
Isolation and Characterization of Wild Type Acetobacter xylinum from Nata de Coco Industry in Surakarta Residency
E R Karuni, A M Sari, A Nursiwi, A P Sanjaya
Starter quality is one of critical factor in nata de coco fermentation. The isolation of nata de coco starter was needed in order to obtain pure isolate. In this research, isolation was conducted to 3 different starters from small nata de coco industries in Surakarta Regency (SME). The isolation produced...
Preliminary Study on the Bacterial Community Structure of Ganoderma Soil Under Oil Palm Plantation
F Hidayat, R Farrasati, I Pradiko, E Listia, M Syarovy, S Rahutomo, Winarna
Basal stem rot (BSR) disease is caused by Ganoderma boninense; it has become a major disease in oil palm plantations over the years and causes a significant yield drop in oil palm plantations, especially in Sumatera. Nowadays, the incidence level of BSR across Sumatera has reached 39% and is predicted...
Analysis of Bacterial Community Structure and Diversity in Traditional Gold Mining Waste Disposal Site by Using a Metabarcoding Approach
Fatimawali, B J Kepel, M A Gani, T E Tallei
The purpose of this study was to evaluate the effect of mercury contamination on bacterial community and structure in the contaminated soil at the mining waste disposal site (sample1) compare with uncontaminated soil (sample2). Mercury level was analyzed using the CVAFS method, and analysis of bacterial...
The Role of Synbiotics (Commercial Product) as a Substitute for Antibiotic Growth Promotor (AGP) in the Performance and Blood Values of Cobb-strain Broilers Challenged with Salmonella enteritidis
J C Ajiguna, V C Prakasita, T E M Nahak, C R Tabbu, C M Santosa, A E T H Wahyuni
Feed is the main requirement for broilers farms. However, continuous use of AGP can cause antibiotic resistance in poultry and humans. The effectiveness of feed additives in preventing some disease agents has not been much scrutinized. Among the diseases that often arise is Salmonellosis. This study...
Isolation and Identification of Mold in Banana Bunches and Their Potential as Bioinoculants to Accelerate Decomposition of Household Organic Waste
L Prihastini, A H Ramelan, P Setyono, Pranoto, A Supriyanto
Large populations and final storage areas cause an increase in organic waste. Efforts are needed to improve waste decomposition. Mold is one of the microorganisms that can break down organic waste. This study aims to 1) isolate and identify molds from 8 banana bunches, 2) calculate mold populations....
Lipid Production from Zygosaccharomyces siamensis AP1 Using Glycerol as a Carbon Source
M. Ilmi, M. Siswontoro
Yeasts are considered as potential lipid producer because they are easy to cultivate, able to grow in high cell densities and can produce high concentrations of lipids. Previously we had isolated a promising lipid producing yeast, Zygosaccharomyces siamensis AP1, from wild honey of Central Sulawesi,...
Effect of Nutmeg (Myristica fragrans Houtt) Leaves Tannin on in Vitro Fermentation Parameters and Methane Mitigation
M Canadianti, L M Yusiati, C Hanim
This research aimed to find out the effect of addition nutmeg (Myristica fragrans Houtt) leaves tannin on in vitro microbial protein, total protozoa, ammonia concentration (NH3), and methane (CH4) production. Materials used in this research were nutmeg (Myristica fragrans Houtt) leaves as a source of...
The Potential of Microbes Isolated from Spent Substrate of Shiitake and Oyster Mushrooms to Induce Resistance Against Early Blight Disease in Tomatoes
N Istifadah, L Herawati
Early blight disease caused by Alternaria solani is an important disease in tomatoes. An environmentally friendly control measure is the use of organic matter, such as spent mushroom substrates (SMS). SMS controls plant diseases primarily through microbial activity that can inhibit the pathogen. The...
Pretreatment of Palm Oil Mill Effluent (POME) Using Lipase and Xylanase to Improve Biogas Production
O M Shafwah, D Suhendar, S Hudiyono
The production process at biogas reactors from Palm Oil Mill Effluent (POME) often faces problems due to limited hydrolysis rates. This limitation occurs due to the formation of mud and lumps which reduce the effective volume of the biogas digester and reduce the potential for biogas produced. The sludge...
Modification and Optimization of Low-cost Medium for Recombinant Alkalothermophilic Xylanase Production from Pichia pastoris KM71
R D Cahyati, S Hudiyono, I Helianti
Xylanase is an enzyme that can degrade xylan into xylooligosaccharides by cleaving to 1, 4-β-D-xylosidic bonds and has high potential in industrial applications. In the previous study, recombinant Pichia pastoris via pPICZ-alpha vector has been constructed, and the yeast produced xylanase originally...
The Role of Probiotics in Suppressing Campylobacter jejuni Infection in Broilers: Performance Parameters
T E M Nahak, V C Prakasita, L N Imanjati, S L Adrenalin, C R Tabbu, A E T H Wahyuni
The use of probiotics as feed additives in farms has been widely known both at a small and large scale. As a substitute for the Antibiotic Growth Promoter (AGP) that has been banned for animal feed, probiotics are used in broilers feeds to improve their performance. Campylobacter jejuni is a type of...
Potential Next-Generation Probiotics Isolated from Romaine Lettuce (Lactuca sativa L. var. longifolia) Fermented Brine
T E Tallei, Fatimawali, A M Sumual, M A Gani, G A V Pollo, A A Adam, J J Pelealu
Nowadays, the search for potential probiotics from lactic acid bacteria continues to develop exclusively for pharmaceutical applications. This study aimed at evaluating the potency of lactic acid bacteria (LAB) isolated from fermented brine of romaine lettuce as next-generation probiotics. The study...
Characterization of Bacteriocin Produced by Bacillus subtilis 11A Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus)
V A Cahya, C Hanim, L M Yusiati
The purpose of this study was to characterize bacteriocins produced by Bacillus subtilis 11A isolated from Indonesian native chicken’s ileum. Characterization of bacteriocins included antimicrobial activity, the stability to temperature, pH, and storage time. Antimicrobial activity was tested against...
Susceptibility Test of Staphylococcus aureus Isolated from Cow Milk, Goat Milk, and Dairy Farm Workers Against Various Antibiotics
C M Santosa, N P Rukmi, F B Lestari, M Wasissa, D A Dewananda, S I O Salasia
Staphylococcus aureus is a Gram-positive bacteria causing pneumonia, mastitis, meningitis, and urinary tract infections. The emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain caused a health problem that needed more attention in both human and animal medicine....
A Potential Yeast Strains for Biological Control of Mosquitoes
D Satwika, V R A Permatasari, G E N Cahyani
Malaria is one of the diseases that have not been successfully eradicated, and are still a threat in many countries. Efforts have been made to eradicate vector-borne diseases, including the use of insecticides and physical approaches, which are proven not effective in overcoming the malaria problem....
Antibacterial Activity of Endophytic Fungi Isolated from Pometia pinnata J. R. Forst & G. Forst Against Escherichia coli
D Prasetyani, R Setyaningsih, A Susilowati
The emerging of antimicrobial resistance become a global concern, encouraging researchers to find out alternative antimicrobial compounds. Endophytic fungi associated with medicinal plants become promising sources because of their ability to produce medical bioactive. This study aimed at bioprospecting...
The Development of Antimicrobial and Food Preservative Agents from the Combination of Emprit Ginger (Zingiber officinale var. amarum) and Nisin
D A Ashari, R Utami, A M Sari, A Nursiwi, A Nissa
Salmonella typhimurium, Eschericia coli, Pseudomonas fluorescense, and Aspergillus niger are common contaminants in food. Microbial contamination in food may lead to food spoilage and foodborne diseases. The development of new antimicrobial agent, especially from natural resources is needed to prevent...
The Ability of Lactobacillus plantarum Bacteria to Denaturate Goat Skin in Producing Gelatin with Different Goat Ages and Fermentation Times
Hasma, E Abustam, R Malaka, MI Said, ZD Zainuddin
Goat skin is rich in protein compounds such as collagen, but it is highly bound to calcium minerals which have the potential to be processed into gelatin, so that for the denaturation process of skin proteins, fermented using L. plantarum. Curing stage on goat skin using chemical acid has done a lot,...
Isolation and Activation of Fresh Fruit Bunch Oil Palm Acetyl Co-A Carboxylase
I. Kresnawaty, D. Santoso, S. Hudiyono
Palm oil has a substantial contribution to national economy, as well as local economy development. At present the productivity of Indonesian oil plam plantation, especially small holderd is relatively low. Efforts to increase productivity can be done through biochemicals approach in this case enzymes...
Characterization of Crude Amylase Activity from Bacteriocin-Producing Lactobacilli
NR Mubarik, HA Surya, Suryani
Bacteriocin-producing Lactobacilli have been successfully isolated from inasua, a fermented fish product from Teon, Tilapia, and Serua, Province of Maluku Indonesia. In addition to producing bacteriocin, Lactobacilli could produce amylase enzymes. This study aims to characterize crude amylase enzyme...
Lactic Acid Bacteria Fermentation of High Protein Feeds: The Effect of Molasses and Incubation Time on Improving Digestibility
P G Kinayang, Z Bachruddin, A Kurniawati
This research aimed to find out the effects of molasses level addition as soluble carbohydrate sources and incubation time towards in vitro digestibility of fermented protein feed. The treatments given to the fermented protein concentrate were the level of molasses of 0, 2, and 4% and an incubation time...
The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride
Pujiati, M W Ardhi, E Muktiani, N K Dewi, N Jadid, E N Prasetyo
This study aims to determine the effect of substrate types on the crude activity of cellulase enzymes from local agricultural waste such as peanut shells, coconut fibers, brans, and teak leaves with the variations of incubation times (day 1, 3, 5, and 7) produced by Trichoderma viride. Enzyme activity...
Lactic Acid Bacteria Fermentation of High Protein Feeds: Effect Storage Time Aerobically and Turmeric (Curcuma Longa) Addition on Quality of Fermented, Fortified and Protected Protein Feed
R Abdiwibowo, Z Bachruddin, A Kurniawati
Protein feed is an essential nutrient for livestock production, but it is easily spoiled if not appropriately handled. This study aimed to determine the effect storage time aerobically and turmeric (Curcuma longa) addition on chemical and physical properties on fermented, fortified, and protected protein...
Bacterial Coating on Seeds as a Potential Application of Bio-inoculants
R Simarmata, Ngadiman, M S Rohman
Seed inoculation with plant growth promoting bacteria (PGPB) is an ideal tool to supply the soil with a high density of beneficial microorganisms. However, viability of microorganisms is a major problem during seed treatment and storage. In this research, seed inoculated by PGPB was conducted to prove...
Microbial Isolations from Olive Ridley Sea Turtle (Lepidochelys olivacea) in Alas Purwo National Park, Indonesia
R Sagasiousman, S Khairani
Infectious diseases in reptiles are one of the biggest causes of morbidity and mortality, related to immunosuppressive factors. Disease in Olive Ridley Sea Turtle arise because ineligible ecosystem in the sea, like bacterial infection. In the world, there are seven species of Sea Turtles, such as: Chelonia...
Effect of Vegetable Oils in Growth of Lactococcus garvieae Isolated from Palm Oil-contaminated Soil and Its Lipase Production
S Sumarsih, S Hadi, Fatimah, SL Bayasud
This research aims to study the effect of vegetable oils in the growth of of Lactococcus garvieae isolated from palm oil-contaminated soil and its active lipase production. The bacteria was grown in medium containing minerals and vegetable oils (virgin olive oil, virgin coconut oil, mustard oil and sunflower...
Remediation of Lead Contaminated Paddy Field Using Ramie Plants Combined with Bioremediation Agents and Inorganic Fertilizer
Sutami, R Rosariastuti, Purwanto
Industrial waste containing heavy metals which used for wetland irrigation will affect the heavy metals content in the soil. The accumulation of heavy metals in the soil may result in reducing microbial activity, soil fertility, soil quality and heavy metals compounds in agricultural products. Agricultural...
Metabolome profile of osmotolerant rhizobacteria under osmotic stress
T A Surya, O Palupi, T Yuwono, Ngadiman, S Wedhastri
An osmotolerant rhizobacteria (Enterococcus flavescens), isolated from the weed rhizosphere, has been subjected to osmotic stress in broth media supplemented with varied concentrations of glucose and sodium chloride (NaCl). The aims of this work was to evaluate the cells’ response and obtain a comprehensive...
Spatial Analysis in Polymerase Chain Reaction for Detection of Corynebacterium diphtheriae Post-outbreak in Samarinda 2018
N Tandirogang, G P D Pratama, Yuniati, I Fikriah, V Abrizal, D E Bulan, Yadi
Diphtheria is an infectious disease caused by the bacterium Corynebacterium diphtheria, which primarily infects the throat and upper airways, and produces a toxin affecting other organs. In severe cases, it causes myocarditis or peripheral neuropathy. In Indonesia, diphtheria has once been an epidemic...
The Use of Pediococcus halophillus FNCC-0032 and Pediococcus acidilactici F-11 to Suppress the Population of Salmonella – Shigella and Enterobacteriaceae in Jambal Roti (Fermented Fish)
M Karyantina, S Anggrahini, T Utami, E S Rahayu
Jambal roti is a fermented product from Manyung fish, which is quite famous in Java. The term ‘jambal roti’ refers to the salting and drying of fish. Manyung fish are easily damaged so that they need to be preserved by salting. The use of 30% salt in traditional production makes the product too salty....
Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov
S Kasmiyati, EBE Kristiani, MM Herawati
Artemisia cina is one of the members of Compositae family that have been identified to display antimicrobial activity. This study aims to examine the ability of wild-type A. cina (KJT genotype) and its polyploid mutant (J genotype) in inhibiting the growth of Gram-positive and Gram-negative bacteria...
Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island
S Margino, Y Mallisa, W Asmara
Utilizing of chlorine-based chemicals in pulp bleaching process causes environmental problem. Utilization of biological agents, such as enzymes for bleaching process is a wise measure to reduce the problem. One of the enzymes that play an important role in pulp bleaching process is xylanase. The previous...