Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

Susceptibility Test of Staphylococcus aureus Isolated from Cow Milk, Goat Milk, and Dairy Farm Workers Against Various Antibiotics

Authors
C M Santosa, N P Rukmi, F B Lestari, M Wasissa, D A Dewananda, S I O Salasia
Corresponding Author
S I O Salasia
Available Online 12 August 2021.
DOI
https://doi.org/10.2991/absr.k.210810.022How to use a DOI?
Keywords
Staphylococcus aureus, Susceptibility test, antibiotics
Abstract

Staphylococcus aureus is a Gram-positive bacteria causing pneumonia, mastitis, meningitis, and urinary tract infections. The emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain caused a health problem that needed more attention in both human and animal medicine. This research aimed to determine the type of antibiotic that was appropriate and effective in treating diseases caused by bacteria in animals and humans. The susceptibility test was carried out by the diffusion method using Mueller Hinton Agar (MHA) and against seven various antibiotic discs. The samples used for this test were 18 Staphylococcus aureus isolated from cow and goat’s milk and a nasal swab of a dairy farmworker. The results of the measured resistance zone formed were interpreted with a standard interpretation table of the Kirby-Bauer. The results showed that 18 Staphylococcus aureus isolates were resistant to ampicillin (100%), oxacillin (83,33%), penicillin-G (100%), tetracycline (72.22%). The isolates showed intermediate susceptibility to erythromycin (61.11%), oxacillin (5,56%), and sensitivity to gentamicin (100%), cefoxitin (77.78%), and oxacillin (11,11%). It was concluded that gentamicin and cefoxitin were still effective in treating diseases caused by Staphylococcus aureus.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
978-94-6239-417-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.210810.022How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - C M Santosa
AU  - N P Rukmi
AU  - F B Lestari
AU  - M Wasissa
AU  - D A Dewananda
AU  - S I O Salasia
PY  - 2021
DA  - 2021/08/12
TI  - Susceptibility Test of Staphylococcus aureus Isolated from Cow Milk, Goat Milk, and Dairy Farm Workers Against Various Antibiotics
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 115
EP  - 118
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.022
DO  - https://doi.org/10.2991/absr.k.210810.022
ID  - Santosa2021
ER  -