Characterization of Crude Amylase Activity from Bacteriocin-Producing Lactobacilli
- 10.2991/absr.k.210810.028How to use a DOI?
- amylolytic index, inasua, MRSA, pH optimum
Bacteriocin-producing Lactobacilli have been successfully isolated from inasua, a fermented fish product from Teon, Tilapia, and Serua, Province of Maluku Indonesia. In addition to producing bacteriocin, Lactobacilli could produce amylase enzymes. This study aims to characterize crude amylase enzyme activity from bacteriocin-producing Lactobacillus plantarum ITN13 and Lactobacillus rhamnosus IN13 based on pH and temperature optimum enzyme activity. Both isolates were grown on media de Mann Rogosa Sharpe Agar (MRSA) + 1% starch and media Nurient Agar (NA) + 1% starch. Amylolytic index (AI) of L. plantarum ITN13 and L. rhamnosus IN13 were 1.33 and 1.00, respectively. Amylase production of L. rhamnosus IN13 maximum at 3 hours incubation was 0.123 (Unit / mg protein) while L. plantarum ITN13 at 6 hours was 0.092 (Unit / mg protein) in the exponential phase, respectively The optimum amylase activity from L. rhamnosus IN13 at 45oC and pH 5, while the optimum of amylase L. plantarum ITN13 at 35oC and pH 6.
- © 2021, the Authors. Published by Atlantis Press.
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- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - NR Mubarik AU - HA Surya AU - Suryani PY - 2021 DA - 2021/08/12 TI - Characterization of Crude Amylase Activity from Bacteriocin-Producing Lactobacilli BT - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019) PB - Atlantis Press SP - 153 EP - 157 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210810.028 DO - 10.2991/absr.k.210810.028 ID - Mubarik2021 ER -