Preliminary Study on the Bacterial Community Structure of Ganoderma Soil Under Oil Palm Plantation

DOI

10.2991/absr.k.210810.010How to use a DOI?

Keywords

Bacterial community structure, Ganoderma, Next generation sequencing, Oil palm

Abstract

Basal stem rot (BSR) disease is caused by Ganoderma boninense; it has become a major disease in oil palm plantations over the years and causes a significant yield drop in oil palm plantations, especially in Sumatera. Nowadays, the incidence level of BSR across Sumatera has reached 39% and is predicted to keep increasing and threatening the sustainability of oil palm plantations between 2050 and 2100. Some researchers believe that Ganoderma is dominant due to the unbalance of the microbial community in the soil ecosystem. This study aims to discover the bacterial community structure in the soil under Ganoderma boninense infection in oil palm plantations. The study was conducted by comparing the soil infected by Ganoderma boninense (G+) and the healthy soil (G-) through the next-generation sequencing (NGS) by Illumina MiSeq. The study shows that the total bacteria of the healthy soil (G-) was 177 times higher than the endemic soil with a total copy number 1.32x108 and 7.44x105, respectively. Acidobacteria was the dominant phyla in the healthy soil (G-), followed by Proteobacteria, and their relative abundance are 31.45% and 29.19%, respectively. On the other hand, the relative abundance of Acidobacteria in the endemic soil (G+) was decreased to 18.73% while Proteobacteria was increased to 38.34%. However, the abundance of these phyla in the endemic soil (G+) is still lower than in the healthy soil (G-). At the level species, the healthy soil (G-) was more diverse than the endemic soil (G+). It shows that the endemic soil is more susceptible to Ganoderma boninense due to its dominance in the soil ecosystems. The 16S rRNA gene sequencing revealed that more than 60% of OTUs had <98% of similarity. It is indicated that some species, both in healthy soil (G-) and endemic soil (G+), under oil palm plantations might be novel species.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - F Hidayat
AU  - R Farrasati
AU  - I Pradiko
AU  - E Listia
AU  - M Syarovy
AU  - S Rahutomo
AU  - Winarna
PY  - 2021
DA  - 2021/08/12
TI  - Preliminary Study on the Bacterial Community Structure of Ganoderma Soil Under Oil Palm Plantation
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 49
EP  - 53
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.010
DO  - 10.2991/absr.k.210810.010
ID  - Hidayat2021
ER  -