Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

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Isolation and Characterization of Wild Type Acetobacter xylinum from Nata de Coco Industry in Surakarta Residency

Authors
E R Karuni, A M Sari, A Nursiwi, A P Sanjaya
Corresponding Author
A M Sari
Available Online 12 August 2021.
DOI
10.2991/absr.k.210810.009How to use a DOI?
Keywords
Isolation, Acetobacter xylinum, Acetic acid bacteria, Characterization
Abstract

Starter quality is one of critical factor in nata de coco fermentation. The isolation of nata de coco starter was needed in order to obtain pure isolate. In this research, isolation was conducted to 3 different starters from small nata de coco industries in Surakarta Regency (SME). The isolation produced 13 isolates which have 2 different shapes, where 8 isolates were rods and 5 were coccobacilli. All of these 13 isolates were Gram negative. Seven isolates have positive catalase and 6 isolates negative. However only one isolate showed a positive result in acetic acid production using CaCO3-etanol (Frateur) medium, ethanol over oxidation using Carr medium and bacterial cellulose production using HS (Hestrin-Scharmm) broth, namely isolate SRG 3A. Based on such result, isolate which has similar characteristics with Actobacter xylinum in references is only 1 isolate, and that is isolate obtained from SME in Sragen, SRG 3A. Both isolate SRG 3A and Actobacter xylinum have rods shape, Gram negative, catalase positive, producing acetic acid, over oxidized ethanol and bacterial cellulose.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
10.2991/absr.k.210810.009
ISSN
2468-5747
DOI
10.2991/absr.k.210810.009How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - E R Karuni
AU  - A M Sari
AU  - A Nursiwi
AU  - A P Sanjaya
PY  - 2021
DA  - 2021/08/12
TI  - Isolation and Characterization of Wild Type Acetobacter xylinum from Nata de Coco Industry in Surakarta Residency
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 44
EP  - 48
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.009
DO  - 10.2991/absr.k.210810.009
ID  - Karuni2021
ER  -