Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

Effect of Vegetable Oils in Growth of Lactococcus garvieae Isolated from Palm Oil-contaminated Soil and Its Lipase Production

Authors
S Sumarsih, S Hadi, Fatimah, SL Bayasud
Corresponding Author
S Sumarsih
Available Online 12 August 2021.
DOI
https://doi.org/10.2991/absr.k.210810.034How to use a DOI?
Keywords
Lactococcus garvieae, palm oil, Lipase
Abstract

This research aims to study the effect of vegetable oils in the growth of of Lactococcus garvieae isolated from palm oil-contaminated soil and its active lipase production. The bacteria was grown in medium containing minerals and vegetable oils (virgin olive oil, virgin coconut oil, mustard oil and sunflower oil) as sole carbon sources. The bacteria growth was measured as optical density at λ=600 nm. The lipase production was determined as lipase activity of crude extract toward ρ-nitrophenyl palmitate as a substrate. The results showed that the vegetable oils used provided the good growth and lipase production of L.garvieae. Even though, olive oil had a great inductive effect on bacterial productivity. The addition of 1% olive oil improved the lipase activity by 135% compared to without olive oil. Crude extract with lipase activity of 56.790 - 66.571 U/mL produced by L. garvieae when cultivated in medium containing 1 - 5% olive oil for 20 hours. Electrophoregram of SDS PAGE and zymographic analysis showed that there were 2 types of lipases produced by L. garvieae, as indicated by the presence of protein bands around 85 and 45 kDa.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
978-94-6239-417-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.210810.034How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - S Sumarsih
AU  - S Hadi
AU  - Fatimah
AU  - SL Bayasud
PY  - 2021
DA  - 2021/08/12
TI  - Effect of Vegetable Oils in Growth of Lactococcus garvieae Isolated from Palm Oil-contaminated Soil and Its Lipase Production
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 195
EP  - 200
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.034
DO  - https://doi.org/10.2991/absr.k.210810.034
ID  - Sumarsih2021
ER  -