Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

The Ability of Lactobacillus plantarum Bacteria to Denaturate Goat Skin in Producing Gelatin with Different Goat Ages and Fermentation Times

Authors
Hasma, E Abustam, R Malaka, MI Said, ZD Zainuddin
Corresponding Author
Hasma
Available Online 12 August 2021.
DOI
https://doi.org/10.2991/absr.k.210810.026How to use a DOI?
Keywords
Lactobacillus plantarum, gelatin, fermentation
Abstract

Goat skin is rich in protein compounds such as collagen, but it is highly bound to calcium minerals which have the potential to be processed into gelatin, so that for the denaturation process of skin proteins, fermented using L. plantarum. Curing stage on goat skin using chemical acid has done a lot, but using biologycal acid with Lactic Acid Bacteria (LAB) Lactobacillus plantarum is still very rare. The presence of lactic acid levels produced by BAL L. plantarum on MRS-Broth media that has been added to goat skin ensures that L. plantarum BAL bacteria can grow well. This research is the initial information to determine the ability of L. plantarum bacteria to grow on goat skin with indicators of bacterial population, lactic acid production, pH and total dissolved protein with different goat ages and fermentation times. This study uses a completely randomized design (CRD) factorial pattern 3 x 3 by treating the age factor of goats (1 year, 2 years and 3 years) and the fermentation time factor (24 hours, 48 hours and 72 hours). L. plantarum bacteria are isolated from milkfish, male goat skin Ettawah Breeds (PE) obtained from the same farm. Each treatment was repeated 4 times. The results showed that the bacterial population experienced rapid growth and a significant increase in the fermentation duration of 24 hours, 48 hours to 72 hours, the number of cells ranged from 8.50±0.45 log10 CFU/mL, 8.81±0.41 log10CFU/mL. 9.34±0.70 log10CFU/mL. From this result, the duration of fermentation is 72 hours at most. Likewise, lactic acid production experienced a significant increase in the 24-hour fermentation duration to 72 hours starting from 1.03±0.17% -1.05± 0.04%. pH at 1 year of age 5.44 ± 0.37 tended to be acidic compared to 2 years and 3 years (5.60±0.16 and 5.60±0.26) which showed the age of 1 year was the highest acidity. Total dissolved protein showed a significant effect on the age of the goat and the duration of fermentation, the longer the fermentation (72 hours), the more dissolved protein (8.80% g/mL ±0.66% g/mL), the lower the age of the goat, the more goat protein dissolved high (1 year) (8.57% ±0.97% g/mL). The most effective growth ability of L. plantarum was obtained at 72 hours fermentation and 1 year old goats showed better quality than skin 1 and 2 years to be used as curing material in gelatin making.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
978-94-6239-417-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.210810.026How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Hasma
AU  - E Abustam
AU  - R Malaka
AU  - MI Said
AU  - ZD Zainuddin
PY  - 2021
DA  - 2021/08/12
TI  - The Ability of Lactobacillus plantarum Bacteria to Denaturate Goat Skin in Producing Gelatin with Different Goat Ages and Fermentation Times
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 138
EP  - 143
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.026
DO  - https://doi.org/10.2991/absr.k.210810.026
ID  - 2021
ER  -