Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

The Potential of Microbes Isolated from Spent Substrate of Shiitake and Oyster Mushrooms to Induce Resistance Against Early Blight Disease in Tomatoes

Authors
N Istifadah, L Herawati
Corresponding Author
N Istifadah
Available Online 12 August 2021.
DOI
https://doi.org/10.2991/absr.k.210810.016How to use a DOI?
Keywords
Alternaria solani, Lentinula edodes, Pleurotus, β-1, 3-glucanase, chitinase
Abstract

Early blight disease caused by Alternaria solani is an important disease in tomatoes. An environmentally friendly control measure is the use of organic matter, such as spent mushroom substrates (SMS). SMS controls plant diseases primarily through microbial activity that can inhibit the pathogen. The objective of this study is to examine the potential of bacteria and fungi isolated from spent substrate of shiitake mushrooms (Lentinula edodes) and oyster mushrooms (Pleurotus sp.) to induce resistance against early blight disease in tomatoes. The isolation from SMS of L. edodes resulted in seven bacterial isolates and four fungal isolates and the isolation from SMS of Pleurotus sp. resulted in five bacterial isolates and two fungal isolates. The isolates were examined for their ability to inhibit early blight disease in tomatoes. The experiment was arranged in randomised complete block design with three replicates. The results showed that the application of the fungal and bacterial isolates in the planting holes inhibited the development of early blight disease in tomatoes by 23.0%–51.6%. The isolates that showed the greatest disease reduction were two bacterial isolates (SB4 and SB7) from spent shiitake substrate. These isolates increased the activities of pathogenesis-related proteins (β-1,3-glucanase and chitinase), indicating the induction of host resistance.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
978-94-6239-417-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.210810.016How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - N Istifadah
AU  - L Herawati
PY  - 2021
DA  - 2021/08/12
TI  - The Potential of Microbes Isolated from Spent Substrate of Shiitake and Oyster Mushrooms to Induce Resistance Against Early Blight Disease in Tomatoes
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 80
EP  - 85
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.016
DO  - https://doi.org/10.2991/absr.k.210810.016
ID  - Istifadah2021
ER  -