Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)

Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov

Authors
S Kasmiyati, EBE Kristiani, MM Herawati
Corresponding Authors
EBE Kristiani, MM Herawati
Available Online 12 August 2021.
DOI
https://doi.org/10.2991/absr.k.210810.039How to use a DOI?
Keywords
Antibacterial activity, metabolite, Artemisia cina Berg ex Poljakov
Abstract

Artemisia cina is one of the members of Compositae family that have been identified to display antimicrobial activity. This study aims to examine the ability of wild-type A. cina (KJT genotype) and its polyploid mutant (J genotype) in inhibiting the growth of Gram-positive and Gram-negative bacteria as well as to determine the concentration of kaemferol, quercetin, and artemisinin as secondary metabolites. Antibacterial activity was tested using Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus). The test of antibacterial activity was conducted using a disk diffusion method with 25, 50, 75 and 100% extract concentrations. Kaemferol, quercetin, and artemisinin contents were measured using High-Performance Liquid Chromatography. The extracts of both KJT and J genotypes indicated an inhibition on Gram-negative and Gram-positive bacteria growth. The antibacterial activity of both polyploid mutant (J) and wild type (KJT) genotypes was showed insignificantly different. The largest bacterial activity was observed in J genotype extract at 50% concentration against B. subtilis with a diameter of inhibition of 14.3 ± 0,60 mm. J genotype extract also showed a higher kaemferol, quercetin and artemisinin concentration compared to KJT genotype.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
Series
Advances in Biological Sciences Research
Publication Date
12 August 2021
ISBN
978-94-6239-417-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/absr.k.210810.039How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - S Kasmiyati
AU  - EBE Kristiani
AU  - MM Herawati
PY  - 2021
DA  - 2021/08/12
TI  - Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov
BT  - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
PB  - Atlantis Press
SP  - 226
EP  - 233
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210810.039
DO  - https://doi.org/10.2991/absr.k.210810.039
ID  - Kasmiyati2021
ER  -