Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov
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- Antibacterial activity, metabolite, Artemisia cina Berg ex Poljakov
Artemisia cina is one of the members of Compositae family that have been identified to display antimicrobial activity. This study aims to examine the ability of wild-type A. cina (KJT genotype) and its polyploid mutant (J genotype) in inhibiting the growth of Gram-positive and Gram-negative bacteria as well as to determine the concentration of kaemferol, quercetin, and artemisinin as secondary metabolites. Antibacterial activity was tested using Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus). The test of antibacterial activity was conducted using a disk diffusion method with 25, 50, 75 and 100% extract concentrations. Kaemferol, quercetin, and artemisinin contents were measured using High-Performance Liquid Chromatography. The extracts of both KJT and J genotypes indicated an inhibition on Gram-negative and Gram-positive bacteria growth. The antibacterial activity of both polyploid mutant (J) and wild type (KJT) genotypes was showed insignificantly different. The largest bacterial activity was observed in J genotype extract at 50% concentration against B. subtilis with a diameter of inhibition of 14.3 ± 0,60 mm. J genotype extract also showed a higher kaemferol, quercetin and artemisinin concentration compared to KJT genotype.
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Cite this article
TY - CONF AU - S Kasmiyati AU - EBE Kristiani AU - MM Herawati PY - 2021 DA - 2021/08/12 TI - Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov BT - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019) PB - Atlantis Press SP - 226 EP - 233 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210810.039 DO - https://doi.org/10.2991/absr.k.210810.039 ID - Kasmiyati2021 ER -