Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island
- 10.2991/absr.k.210810.040How to use a DOI?
- Xylanase purification, Aspergillus tamari TKM-24
Utilizing of chlorine-based chemicals in pulp bleaching process causes environmental problem. Utilization of biological agents, such as enzymes for bleaching process is a wise measure to reduce the problem. One of the enzymes that play an important role in pulp bleaching process is xylanase. The previous conducted research found out xylanolyitic fungi and determined for while as Aspergillus tamari TKM-24. The purpose of research was to purify xylanase enzyme t. The research methods were partial optimization of xylanase production and purification its xylanase Aspergillus tamari TKM-24. Partial optimization including environmental factors (pH, agitation, temperature, airation), inoculum, substrate concentration, and time incubation. Purification steps of xylanase were precipitation by ammonium sulfate and gel filtration on sephadex G-75. Characterization of purified xylanase consisted of molecular weight, effect of pH and temperature to xylanase activity. The results showed that optimization of xylanase production was obtained by inoculum concentration 1 % (v/v), substrate xylan concentration 1% (w/v), pH 5 and incubation time for 4 days. Xylanase showed a single band on SDS-PAGE analysis with a moleculer mass 25 kDa and was purified 10,2 - fold by Spadex G-75. The Km and Vmax values were 24,4 mg/ml and 1520 μmol/ml/min for oat spelt xylan, respectively. The Xylanase was clasified as termotolerant which had optimal temperature was around 55o C and pH value 6.0.
- © 2021, the Authors. Published by Atlantis Press.
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Cite this article
TY - CONF AU - S Margino AU - Y Mallisa AU - W Asmara PY - 2021 DA - 2021/08/12 TI - Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island BT - Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019) PB - Atlantis Press SP - 234 EP - 244 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210810.040 DO - 10.2991/absr.k.210810.040 ID - Margino2021 ER -