Outer Membrane Protein (OMP) Profiles of Brucella abortus Local Isolate by SDS-PAGE Procedure

DOI

10.2991/absr.k.220309.006How to use a DOI?

Keywords

Brucella abortus local isolate; OMP; SDS-PAGE

Abstract

Brucella abortus is a Gram-negative, facultative intracellular bacteria of the genus Brucella that common cause of brucellosis in cattle. The Outer Membrane Protein (OMP) is an essential organelle of Gram-negative bacteria to fulfil several some many tasks and is crucial as a protective antigen. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a universal method for molecular weight separation and analysis of proteins. This study aimed to determine the profile Outer Membrane Protein (OMP) band composition of Brucella abortus local isolate. The B. abortus isolate is a collection of Department of Agriculture, Research and Technology Center for Isotopes and Radiation Application-BRIN), isolated from the hygroma fluid in the case field. N - Lauroylsarcosine (Sarkosyl) methods used to extract outer membrane bacteria. OMP B. abortus was measured protein molecular weight by SDS-PAGE and standard molecular weight of 10 – 250 kDa. This study showed that the profile Outer membrane Proteins (OMP) Brucella abortus local isolate had eight bands there were 16.7-kDa, 18-kDa, 26.6-kDa, 33-kDa, 42-kDa, 55-kDa, 72-kDa, and 91.3-kD.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Tri Handayani
AU  - Dadang Priyoatmojo
AU  - Afi Candra Trinugraha
PY  - 2022
DA  - 2022/03/29
TI  - Outer Membrane Protein (OMP) Profiles of Brucella abortus Local Isolate by SDS-PAGE Procedure
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 28
EP  - 31
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.006
DO  - 10.2991/absr.k.220309.006
ID  - Handayani2022
ER  -