Addition of Trehalose of Duck Egg Yolk-Tris as an Extender Medium on Buffalo Frozen Semen

Tinda Afriani; Yolanda Marta Pela; Jaswandi; Elly Roza; Adisti Rastosari; Anna Farhana

doi:10.2991/absr.k.220309.005

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Addition of Trehalose of Duck Egg Yolk-Tris as an Extender Medium on Buffalo Frozen Semen

Authors

Tinda Afriani¹^{, *}, Yolanda Marta Pela¹, Jaswandi², Elly Roza¹, Adisti Rastosari¹, Anna Farhana²

¹Department of Livestock Production, Faculty of Animal Science, Andalas University, Padang, 25163 West Sumatera, Indonesia.

²Department of Biotechnology and Reproduksi, Faculty of Animal Science, Andalas University, Padang, 25163 West Sumatera, Indonesia

^*Corresponding author. Email: tindaafriani@ansci.unand.ac.id

Corresponding Author

Tinda Afriani

Available Online 29 March 2022.

DOI: 10.2991/absr.k.220309.005 How to use a DOI?
Keywords: Buffalo; egg yolk-tris; frozen semen; trehalose
Abstract: This study aimed to determine the effect of adding trehalose of duck egg yolk-tris extender medium on the quality of frozen semen at buffalo. The design used was experimental a Randomized Block Design (RBD) method with 4 treatments and 4 replications as a group. The addition of trehalose (T) with several concentrations in the extender of tris duck-egg yolk as the first treatment, namely the control consisted of egg yolk tris 80% + 20% + 0% trehalose (T0), the second treatment 0.5% (T1), the third treatment % (T2) and the fourth treatment 1.5% (T3). The observed variables included: motility, viability, abnormality, and membrane integrity. The results of this study showed that the addition of trehalose of duck egg yolk-tris had a non-significantly different effect (P>0.05) on the percentage of spermatozoa motility with the average successively at T0, T1, T2 and T3, namely 25.00±5.80. %, 27.50±5.00%, 30.00±8.17%, 32.50±5.00%, the effect was very significant (P<0.01) on the percentage of spermatozoa viability and membrane integrity, with the mean successively at treatment T0 that is 42.25±2.60%, 43.25±2.90%, T1 44.00±2.68%, 46.00±2.40, T2 46,63±1.11%, 48.25±1.26%, T3 48.25±1.50%, 49.50±2.39%, significantly different effects (P<0.05) on spermatozoa abnormality with the mean successively at T0, T1, T2 and T3 that is 12.38±1.66%, 18.00±3.24%, 19.63±9.07%, 25.50±9.06%. The results of the study concluded that the addition of trehalose to the tris of duck egg yolks could maintain the motility, viability, membrane integrity of spermatozoa, but the addition of trehalose was unable to maintain the abnormality of Buffalo spermatozoa.
Copyright: © 2022 The Authors. Published by Atlantis Press International B.V.
Open Access: This is an open access article under the CC BY-NC license.

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Volume Title: Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series: Advances in Biological Sciences Research
Publication Date: 29 March 2022
ISBN: 10.2991/absr.k.220309.005
ISSN: 2468-5747
DOI: 10.2991/absr.k.220309.005 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC license.

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ris enw bib

TY  - CONF
AU  - Tinda Afriani
AU  - Yolanda Marta Pela
AU  - Jaswandi
AU  - Elly Roza
AU  - Adisti Rastosari
AU  - Anna Farhana
PY  - 2022
DA  - 2022/03/29
TI  - Addition of Trehalose of Duck Egg Yolk-Tris as an Extender Medium on Buffalo Frozen Semen
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 23
EP  - 27
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.005
DO  - 10.2991/absr.k.220309.005
ID  - Afriani2022
ER  -

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