Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016)

Research on the Effects of 5-HT1A Receptor in Hippocampal CA1 Region in the Cognition of PTSD Rats

Authors
Ling Lin, Guoliang Liu, Yinpo Zhang
Corresponding Author
Ling Lin
Available Online February 2017.
DOI
10.2991/meita-16.2017.81How to use a DOI?
Keywords
PTSD, Hippocampal, 5-HT1A Receptor, Spatial Memory
Abstract

Investigate the expression of 5-HT1A receptor in CA1 area of hippocampus of posttraumatic stress disorder (PTSD) rats and to explore the function of 5-HT1A receptor in the cognitive behavior of PTSD rats. Methods:72 healthy adult SD rats were randomly divided into 4 groups: A (control group), B(model group), C(control +agonist group )and D(model + agonist group), with 18 rats in each group. The rats in B and C groups were constructed with continuous single stimulation (SPS) to construct the PTSD model rats. 2 days after the model was completed, the CA1 of the hippocampus was located under the Stereotaxic instrument. The rats of B and C groups were injected with 5-HT1A receptor agonist 8-OH-DPAT, A and B group rats were injected with equal amount of normal saline, and the dose was 0.3mg/kg. Morris water maze(MWM)was used to test the learning and memory ability in different groups of rats, The expression of 5-HT1A and 5-HT1A protein in hippocampus was examined by immunohistochemistry and immunofluorescence and Western Blot. Results:It was showed in the MWM experiment that the latency of rats in searching for the underwater platform in group C rats was significantly shorter compared with the group A(P<0.01), B and C group were increased(P<0.01). Compared with group B, group D rats escape the latency was significantly prolonged(P<0.01). The expression of 5-HT1A receptor in CA1 region of hippocampal by immunohistochemistry and immunofluorescence and Western Blot experiments all showed that, Compared with the group A, the other 3 groups were significantly increased(P<0.01), Compared with the group B, the group D was significantly increased (P<0.01), neverthelessly, there was no significant difference between the B group and the C group (P> 0.05). Conclusion:The spatial memory impairment in rats with PTSD maybe associated with the increase of the expression of 5-HT1A receptor in the CA1 region of hippocampus.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016)
Series
Advances in Engineering Research
Publication Date
February 2017
ISBN
978-94-6252-304-3
ISSN
2352-5401
DOI
10.2991/meita-16.2017.81How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Ling Lin
AU  - Guoliang Liu
AU  - Yinpo Zhang
PY  - 2017/02
DA  - 2017/02
TI  - Research on the Effects of 5-HT1A Receptor in Hippocampal CA1 Region in the Cognition of PTSD Rats
BT  - Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016)
PB  - Atlantis Press
SP  - 390
EP  - 395
SN  - 2352-5401
UR  - https://doi.org/10.2991/meita-16.2017.81
DO  - 10.2991/meita-16.2017.81
ID  - Lin2017/02
ER  -