Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

DOI

10.2991/mseee-17.2017.31How to use a DOI?

Keywords

low-frequency and low-power ultrasound, hydroxyapatite, apoptosis.

Abstract

The exploration of the apoptosis for HepG2 cells induced by low-frequency and low-power ultrasound combined with hydroxyapatite, provides data base for future clinical treatment. In this research, we selected the HepG2 cells model. The experiences were divided into four groups: control group, ultrasound group, hydroxyapatite group, and ultrasound + hydroxyapatite group. Ultrasound group and control group replaced the normal culture medium, hydroxyapatite group and ultrasound + hydroxyapatite group replaced culture medium containing hydroxyapatite. The control group and hydroxyapatite group were not treated, while ultrasound group and ultrasound + hydroxyapatite group were treated by ultrasound with different parameters (f= 20 kHz; P=0.1, 0.2, 0.3w/cm2; t=30, 60, 90, 120, 150, 180s; T= 0, 12, 24, 48, 72h). MTT test and flow cytometry detected the inhibitory rate of the cells, cell apoptosis and cell cycle. MTT experiment results show that ultrasound with different parameters stimulates the cell model, which leads to the difference of the inhibition rate of cell growth. Among them, the inhibition rate of cell growth reached the top value (71.726±0.677)% with the treatment of f=20 kHz, P =0.2w/cm2,t=90s,T=48h. There was a statistical significance compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05).Under such condition, the apoptosis rate of HepG2 cells was a statistically significant change compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05). During the cell cycle assay, the ultrasonic + hydroxyapatite group also showed obvious G2/M phase block. Low frequency and low-power ultrasound with Hydroxyapatite induced the apoptosis in human HepG2 cells.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chen Chen
AU  - Tian Xu
AU  - Weiwei Wang
AU  - Li Li
AU  - Wei Wu
PY  - 2017/08
DA  - 2017/08
TI  - Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite
BT  - Proceedings of the 2017 International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2017)
PB  - Atlantis Press
SP  - 164
EP  - 168
SN  - 2352-5401
UR  - https://doi.org/10.2991/mseee-17.2017.31
DO  - 10.2991/mseee-17.2017.31
ID  - Chen2017/08
ER  -