Construction of a Plant Binary Expression Vector for the Potato Host Factor Genes StTOM1 and StTOM3

DOI

10.2991/msmee-17.2017.66How to use a DOI?

Keywords

Potato, StTOM1, StTOM3, Plant binary expression vector.

Abstract

In this article, we designed PCR primers according to the cDNA sequence of potato host factor genes StTOM1 and StTOM3 and the restriction enzyme sites of the intermediate vector pUCCRNAi to gain two RNA interference segments, which length were 272bp and 182bp, and then connected to the vector pUCCRNAi from two directions simultaneously. After the validation of sequencing and digestion, the bidirectional RNA interference segment with intron was digested from pUStT1-StT3-dRi(±) and connected to pBI121, thus the plant binary expression vector pBIStT1-StT3-dRi(±) was constructed.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jingjing Song
PY  - 2017/05
DA  - 2017/05
TI  - Construction of a Plant Binary Expression Vector for the Potato Host Factor Genes StTOM1 and StTOM3
BT  - Proceedings of the 2017 2nd International Conference on Materials Science, Machinery and Energy Engineering (MSMEE 2017)
PB  - Atlantis Press
SP  - 313
EP  - 317
SN  - 2352-5401
UR  - https://doi.org/10.2991/msmee-17.2017.66
DO  - 10.2991/msmee-17.2017.66
ID  - Song2017/05
ER  -