Proceedings of the 2017 2nd International Conference on Materials Science, Machinery and Energy Engineering (MSMEE 2017)

Construction of a Plant Binary Expression Vector for the Potato Host Factor Genes StTOM1 and StTOM3

Authors
Jingjing Song
Corresponding Author
Jingjing Song
Available Online May 2017.
DOI
https://doi.org/10.2991/msmee-17.2017.66How to use a DOI?
Keywords
Potato, StTOM1, StTOM3, Plant binary expression vector.
Abstract

In this article, we designed PCR primers according to the cDNA sequence of potato host factor genes StTOM1 and StTOM3 and the restriction enzyme sites of the intermediate vector pUCCRNAi to gain two RNA interference segments, which length were 272bp and 182bp, and then connected to the vector pUCCRNAi from two directions simultaneously. After the validation of sequencing and digestion, the bidirectional RNA interference segment with intron was digested from pUStT1-StT3-dRi(±) and connected to pBI121, thus the plant binary expression vector pBIStT1-StT3-dRi(±) was constructed.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2017 2nd International Conference on Materials Science, Machinery and Energy Engineering (MSMEE 2017)
Series
Advances in Engineering Research
Publication Date
May 2017
ISBN
978-94-6252-346-3
ISSN
2352-5401
DOI
https://doi.org/10.2991/msmee-17.2017.66How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Jingjing Song
PY  - 2017/05
DA  - 2017/05
TI  - Construction of a Plant Binary Expression Vector for the Potato Host Factor Genes StTOM1 and StTOM3
BT  - Proceedings of the 2017 2nd International Conference on Materials Science, Machinery and Energy Engineering (MSMEE 2017)
PB  - Atlantis Press
SP  - 313
EP  - 317
SN  - 2352-5401
UR  - https://doi.org/10.2991/msmee-17.2017.66
DO  - https://doi.org/10.2991/msmee-17.2017.66
ID  - Song2017/05
ER  -