Investigation of OMNIgene·SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assays in Uganda

C.D. Kelly-Cirino; E. Musisi; P. Byanyima; S. Kaswabuli; A. Andama; A. Sessolo; I. Sanyu; J. Zawedde; P.S. Curry; L. Huang

doi:10.1016/j.jegh.2017.04.001

<Previous Article In Issue

Next Article In Issue>

Volume 7, Issue 2, June 2017, Pages 103 - 109

Investigation of OMNIgene·SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assays in Uganda

Authors

C.D. Kelly-Cirino^a^{, *}^{, 1}^,casseycirino@gmail.com, E. Musisi^b^{, 1}^,emusisi@idrc-uganda.org, P. Byanyima^b^,pbyanyima@idrc-uganda.org, S. Kaswabuli^b^,skaswabuli@idrc-uganda.org, A. Andama^b^,andama.alf@gmail.com, A. Sessolo^b^,asessolo@idrc-uganda.org, I. Sanyu^b^,singvar@idrc-uganda.org, J. Zawedde^b^,jzawedde@idrc-uganda.org, P.S. Curry^a^,pat.curry@dnagenotek.com, L. Huang^c^,Laurence.Huang@ucsf.edu

^aDNA Genotek, 500 Palladium Dr., Suite 3000, Ottawa, Ontario K2V 1C2, Canada

^bInfectious Diseases Research Collaboration Kampala, 2C Nakasero Hill Road, PO Box 7475, Kampala, Uganda

^cUniversity of California San Francisco, HIV, Infectious Diseases, and Global Medicine Division and Division of Pulmonary and Critical Care Medicine - Ward 84, San Francisco General Hospital, 995 Potrero Avenue, San Francisco, CA 94110, USA

¹

C.D. Kelly-Cirino and E. Musisi are equally contributing co-first authors.

^*Corresponding author.

Corresponding Author

C.D. Kelly-Cirinocasseycirino@gmail.com

Available Online 13 April 2017.

DOI: 10.1016/j.jegh.2017.04.001 How to use a DOI?
Keywords: MGIT; Molecular detection; Mycobacterium tuberculosis; Preservation; Solid culture; Specimen transport medium
Abstract: OMNIgene·SPUTUM (OM-S) is a sample transport reagent designed to work with all tuberculosis diagnostics while eliminating the need for cold chain. OM-S-treated sputum samples were assayed in several tests after multiday holds. Raw sputa from 100 patients underwent direct smear microscopy, were manually split and assigned to the OM-S group [OM-S added at collection (no other processing required) and tested after 0- to 5-day holds at room temperature] or standard-of-care (SOC) group (NaOH/N-acetyl l-cysteine decontamination, all tested on day of collection). Concentrated smear microscopy, Lowenstein Jensen (LJ) culture, and mycobacteria growth indicator tube (MGIT) culture were performed. For patients with negative direct smear, a second sample was split, with SOC (raw sputum) and OM-S portions (sediment) tested in the Xpert MTB/RIF (Xpert) assay. OM-S group and SOC group results were strongly concordant on all four tests [range, 89% (MGIT)–97% (Xpert)]. OM-S MGIT, LJ, and Xpert tests were in statistical agreement with SOC MGIT as reference. OM-S specimens had lower culture contamination rates (3% vs. 10% LJ; 2% vs. 5% MGIT) but required, on average, 5.6 additional days to become MGIT-positive. The findings suggest that samples held/transported in OM-S are compatible with smear microscopy, LJ or MGIT culture, and Xpert, and perform comparably to fresh sputum samples. Larger feasibility studies are warranted.
Open Access: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Download article (PDF)
View full text (HTML)

<Previous Article In Issue

Next Article In Issue>

Journal: Journal of Epidemiology and Global Health
Volume-Issue: 7 - 2
Pages: 103 - 109
Publication Date: 2017/04/13
ISSN (Online): 2210-6014
ISSN (Print): 2210-6006
DOI: 10.1016/j.jegh.2017.04.001 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Cite this article

ris enw bib

TY  - JOUR
AU  - C.D. Kelly-Cirino
AU  - E. Musisi
AU  - P. Byanyima
AU  - S. Kaswabuli
AU  - A. Andama
AU  - A. Sessolo
AU  - I. Sanyu
AU  - J. Zawedde
AU  - P.S. Curry
AU  - L. Huang
PY  - 2017
DA  - 2017/04/13
TI  - Investigation of OMNIgene·SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assays in Uganda
JO  - Journal of Epidemiology and Global Health
SP  - 103
EP  - 109
VL  - 7
IS  - 2
SN  - 2210-6014
UR  - https://doi.org/10.1016/j.jegh.2017.04.001
DO  - 10.1016/j.jegh.2017.04.001
ID  - Kelly-Cirino2017
ER  -

download .riscopy to clipboard