Journal of Epidemiology and Global Health

In Press, Corrected Proof, Available Online: 10 January 2020

Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India

Authors
Rakesh Yadav1, #, Pankaj Vaidya2, #, Joseph L Mathew2, Sanjay Verma2, Rajiv Khaneja3, Priyanka Agarwal4, Pankaj Kumar5, Meenu Singh2, Sunil Sethi1, *
1Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
2Pediatric Medicine, Advanced Pediatric Center, Postgraduate Institute of Medical Education and Research, Chandigarh, India
3State TB Cell, Sector 34, Chandigarh, India
4WHO Country Office for India, New Delhi, India
5Department of Paediatrics, Govt Medical College, Chandigarh, India
#

First two authors are joint first author.

*Corresponding author. Email: sunilsethi10@hotmail.com
Corresponding Author
Sunil Sethi
Received 7 June 2019, Accepted 7 December 2019, Available Online 10 January 2020.
DOI
https://doi.org/10.2991/jegh.k.191215.002How to use a DOI?
Keywords
Xpert MTB/RIF, pediatric tuberculosis, diagnosis, India
Abstract

Tuberculosis (TB) diagnosis in children still remains a challenge in developing countries. We analyze the performance of Xpert MTB/RIF assay for the diagnosis of pediatric TB under programmatic conditions. We retrospectively analyzed the performance of Xpert MTB/RIF assay from February 2016 to March 2018. A total 2678 samples from TB suspects below 14 years were received in the laboratory and were frontline tested by Xpert MTB/RIF assay according to the manufacturer’s instructions. If sample was sufficient, the smear microscopy and culture were performed as per standard World Health Organization’s guidelines. The smears and cultures were performed in 2178 and 588 samples, respectively. Among 2678 samples, 68 were rejected, Xpert MTB/RIF assay was positive in 357/2610 (13.6%) cases, while the smear was positive in 81/2178 (3.3%) cases. The sensitivity of smear and Xpert MTB/RIF when compared with culture was 24.6% (14.1–37.8%) and 81% (68.6–90.1%), respectively. The diagnostic accuracy of Xpert MTB/RIF and smear was 97.1% and 92.2%, respectively. Thirty samples (8.5%) were detected as rifampicin resistance by Xpert MTB/RIF assay. The Xpert MTB/RIF increased the detection rate up to fourfold when compared with smear microscopy. Xpert MTB/RIF assay is the most rapid, sensitive, and specific method for microbiological confirmation and rifampicin resistance detection in pediatric tuberculosis.

Copyright
© 2020 Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
Journal of Epidemiology and Global Health
Publication Date
2020/01
ISSN (Online)
2210-6014
ISSN (Print)
2210-6006
DOI
https://doi.org/10.2991/jegh.k.191215.002How to use a DOI?
Copyright
© 2020 Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Rakesh Yadav
AU  - Pankaj Vaidya
AU  - Joseph L Mathew
AU  - Sanjay Verma
AU  - Rajiv Khaneja
AU  - Priyanka Agarwal
AU  - Pankaj Kumar
AU  - Meenu Singh
AU  - Sunil Sethi
PY  - 2020
DA  - 2020/01
TI  - Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India
JO  - Journal of Epidemiology and Global Health
SN  - 2210-6014
UR  - https://doi.org/10.2991/jegh.k.191215.002
DO  - https://doi.org/10.2991/jegh.k.191215.002
ID  - Yadav2020
ER  -