Comparison of Fixed Range Method and Levey-Jennings Method Used in ELISA Detection Anti-HBc
- 10.2991/icaset-18.2018.10How to use a DOI?
- Fixed range method, Enzyme linked immunosorbent assay, Internal quality control, Levey-Jennings quality control
In this paper aims at using the two different methods (fixed range method and Levey-Jennings method) to study the internal quality control of qualitative ELISA detection anti-HBc and exploring the difference, applicability, effectiveness of the two methods. Method -- the author collects the internal quality control data of hepatitis B core antibody during the 16 months (from October 2015 to February 2017). Fixed range method is used. X2 is upper control limit and X1 is lower control limit. Cut-off value adopts one of the lines. ELISA method detection is two half-and-half. If S/CO≥1 is positive, 1 is the lower limit of week positive control product. If S/CO<1 is positive, 1 is upper limit of week positive control product. When the quality control product is stable, X+3SD or X-3SD of S/CO value shall be included into Levey-Jennings quality control chart. If the deviation % is between X1 and X2, it is in control. Otherwise it is out of control. Result -- two groups of data show difference in the two different methods, fixed range method and Levey-Jennings method. The two methods have the similar out-of-control ratio in negative control and positive control. But fixed range method is better than Levey-Jennings method in weak positive control. It has the statistical significance. Conclusion -- when the two methods are used for anti-HBc detection, fixed range method and Levey-Jennings method show difference in weak positive true out-of-control ratio. Fixed range method is more obvious, sensitive and effective.
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Deyi Ju AU - Jiandong Wang AU - Xiaomei Zhao PY - 2018/04 DA - 2018/04 TI - Comparison of Fixed Range Method and Levey-Jennings Method Used in ELISA Detection Anti-HBc BT - Proceedings of the 2018 8th International Conference on Applied Science, Engineering and Technology (ICASET 2018) PB - Atlantis Press SP - 49 EP - 53 SN - 2352-5401 UR - https://doi.org/10.2991/icaset-18.2018.10 DO - 10.2991/icaset-18.2018.10 ID - Ju2018/04 ER -