Proceedings of the 2016 International Conference on Engineering and Technology Innovations

Enzyme properties and thermal stability of horseradish peroxidase (HRP-DL)

Authors
Te Wang, Jing Tian, Tingting Li, Jian Li
Corresponding Author
Te Wang
Available Online March 2016.
DOI
https://doi.org/10.2991/iceti-16.2016.40How to use a DOI?
Keywords
Horseradish peroxidase, thermal stability, protective agent, stress resistance, Ectione
Abstract
Horseradish peroxidase (HRP) is widely used in biological engineering, wastewater treatment, enzyme-linked immunosorbent assay and other fields. It is great significance to study its enzyme properties, stress resistance and protective agent. In order to provide good quality HRP, and protection method of HRP thermal stability, horseradish peroxidase (HRP-DL) was extracted and purified from horseradish which was collected from Dalian, China in this paper. It was investigated that the enzyme characterization, thermal stability, thermal protection from thermal stability protection agents of HRP-DL. The optimum temperature of HRP-DL was 30 °C, the optimum pH of HRP-DL was 5.5. HRP-DL enzyme activity was inhibited significantly by NaCl. HRP-DL was activated by Ca2+, Mn2+, Zn2+, Pb2+, Mg2+, Go2+, Fe3+, and inhibited by K+, Cu2+, Hg2+, Ag+ and Fe2+ in different degree. When HRP-DL was processed at the temperature greater than or equal to 35 °C, the residual enzyme activity was reduced. The HRP-DL residual enzyme activity increase 49.3% when added 1 mmol/L Ectoine and heat treatment at 60 °C for 15 min. The study of HRP-DL enzyme characterization provided the basis for its practical application. Ectoine as HRP-DL thermally stable protective agent has advantages such as the use of less, the effect is significant.
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Proceedings
2016 International Conference on Engineering and Technology Innovations
Part of series
Advances in Engineering Research
Publication Date
March 2016
ISBN
978-94-6252-170-4
ISSN
2352-5401
DOI
https://doi.org/10.2991/iceti-16.2016.40How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Te Wang
AU  - Jing Tian
AU  - Tingting Li
AU  - Jian Li
PY  - 2016/03
DA  - 2016/03
TI  - Enzyme properties and thermal stability of horseradish peroxidase (HRP-DL)
BT  - 2016 International Conference on Engineering and Technology Innovations
PB  - Atlantis Press
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceti-16.2016.40
DO  - https://doi.org/10.2991/iceti-16.2016.40
ID  - Wang2016/03
ER  -