Study on the enzymatic properties of Nattokinase

DOI

10.2991/icimm-15.2015.48How to use a DOI?

Keywords

Nattokinase;Enzymatic Properties;Salting Out

Abstract

The fractionation by (NH4)2SO4 of nattokinase was studied,the (NH4)2SO4saturation of 30%was selected to remove impurity protein, (NH4)2SO4 saturation of 60% was seleted to precipitate nattokinase.The nattokinase was purified bysalting out method to obtain the crude enzyme solution,the enzymatic properties of nattokinase were studied.The nattokinase was long time no inactivation under the temperature of 40 ,nattokinase was preserved for 5 hours at the temperature of 37 ,the enzyme activity was not affected.The activity of nattokinase was stable in the range of pH6-9,when the pH is 3,the activity of nattokinase was very low,but when pH returned to 7,enzyme activity recovery.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yingge Yang
PY  - 2015/07
DA  - 2015/07
TI  - Study on the enzymatic properties of Nattokinase
BT  - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials
PB  - Atlantis Press
SP  - 251
EP  - 255
SN  - 2352-5401
UR  - https://doi.org/10.2991/icimm-15.2015.48
DO  - 10.2991/icimm-15.2015.48
ID  - Yang2015/07
ER  -