Study on Nattokinase-Extraction with two-phase aqueous system

DOI

10.2991/icimm-15.2015.47How to use a DOI?

Keywords

Nattokinase;Two-phase Aqueous Extration;Distribution Behavior

Abstract

The extraction of nattokinase with two-phase aqueous system was studied.Distribution behavior of nattokinase with PEG400/(NH4)2SO4 and PEG6000/MgSO4 two-phase aqueous systems were studied respectively. PEG400/(NH4)2SO4 two-phase aqueous system is suitable for the extraction of nattokinase. Various factors affecting the extraction of nattokinase with PEG400/(NH4)2SO4 two-phase aqueous system were studied. The mass fraction of PEG400 was determined to be 24%, The mass fraction of (NH4)2SO4 was determined to be 27%, the addition of crude enzyme was determined to be 15%. Under these conditions,purification of nattokinase reached 2.11.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yingge Yang
PY  - 2015/07
DA  - 2015/07
TI  - Study on Nattokinase-Extraction with two-phase aqueous system
BT  - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials
PB  - Atlantis Press
SP  - 246
EP  - 250
SN  - 2352-5401
UR  - https://doi.org/10.2991/icimm-15.2015.47
DO  - 10.2991/icimm-15.2015.47
ID  - Yang2015/07
ER  -