Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials

Study on Accurate Determination of Volatile Fatty Acids in Rumen Fluid by Capillary Gas Chromatography

Authors
Cheng Luo, Suyi Cai, Linyan Jia, Xiang Tang, Ruinan Zhang, Gang Jia, Hua Li, Jiayong Tang, Guangmang Liu, Caimei Wu
Corresponding Author
Cheng Luo
Available Online July 2015.
DOI
https://doi.org/10.2991/icimm-15.2015.73How to use a DOI?
Keywords
Capillary gas chromatography; Rumen fluid; Volatile fatty acids; Internal standard method
Abstract
In order to obtain accurate results of volatile fatty acids (VFAs) in rumen fluid by capillary gas chromatography (GC), a pretreatment method was firstly conducted by dissolving VFAs in the methanol. An internal standard method (ISM) with crotonic acid as the internal standard was adopted in the detection. The results showed that the reproducibility of VFA (acetic acid, propanoic acid and butyric acid) was enhanced remarkably with methanol added as the solvent. The reproducibility was dependent on both of the VFA concentration and methanol amount. In conclusion, methanol solvent was more suitable than aqueous solvent for VFA accurate detection with ISM by capillary GC.
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This is an open access article distributed under the CC BY-NC license.

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Volume Title
Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials
Series
Advances in Engineering Research
Publication Date
July 2015
ISBN
978-94-62520-88-2
ISSN
2352-5401
DOI
https://doi.org/10.2991/icimm-15.2015.73How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Cheng Luo
AU  - Suyi Cai
AU  - Linyan Jia
AU  - Xiang Tang
AU  - Ruinan Zhang
AU  - Gang Jia
AU  - Hua Li
AU  - Jiayong Tang
AU  - Guangmang Liu
AU  - Caimei Wu
PY  - 2015/07
DA  - 2015/07
TI  - Study on Accurate Determination of Volatile Fatty Acids in Rumen Fluid by Capillary Gas Chromatography
BT  - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials
PB  - Atlantis Press
SP  - 386
EP  - 391
SN  - 2352-5401
UR  - https://doi.org/10.2991/icimm-15.2015.73
DO  - https://doi.org/10.2991/icimm-15.2015.73
ID  - Luo2015/07
ER  -