Proceedings of the International Conference on Innovation and Technology (ICIT 2021)

Comparative Study on the Extraction and Quantification of Polyphenols from Moringa oleifera Leaves Using Maceration with Pulsed Electric Field (PEF) as Pretreatment and Microwave-Assisted Extraction (MAE)

Authors
Sukardi*, Puput Safitri, Hendrix Yulis Setyawan
Department of Agro-industrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, Indonesia
*Corresponding author. Email: sukardi@ub.ac.id
Corresponding Author
Sukardi
Available Online 23 December 2021.
DOI
10.2991/aer.k.211221.019How to use a DOI?
Keywords
Antioxidant activity; Extraction; Maceration; Moringa leaves; Pulsed Electric Field
Abstract

Moringa oleifera leaves are rich in nutrients and bioactive compounds such as polyphenols which can be used as an antioxidant. Polyphenols of Moringa leaves can be extracted using conventional and non-conventional methods. This study compared maceration with Pulsed Electric Field (PEF) pretreatment and MAE method on extracting polyphenols from Moringa leaves. The performance of those methods was assessed based on the yield extracts, total polyphenol content (TPC), and antioxidant activity (IC50). The results showed that different methods significantly affected TPC and antioxidant activity. Maceration with PEF pretreatments obtained extracts with the highest TPC value (179.11 mg GAE/g) and antioxidant activity (33.56 ppm). In comparison, the MAE method produced TPC and antioxidant activity values of 22.64 mg GAE/g and 54.59 ppm, respectively. The yield extracts did not show differences significantly. Maceration with PEF pretreatment and MAE obtained the yield extract of 12.62% and 13.16%, respectively. Based on the results, it can be seen that maceration with PEF pretreatment has a better ability to increase TPC and antioxidant activity compared to the MAE method. Maceration with PEF pretreatment can increase TPC up to 7.9 times and antioxidant activity up to 1.6 times.

Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Innovation and Technology (ICIT 2021)
Series
Advances in Engineering Research
Publication Date
23 December 2021
ISBN
978-94-6239-493-3
ISSN
2352-5401
DOI
10.2991/aer.k.211221.019How to use a DOI?
Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Sukardi
AU  - Puput Safitri
AU  - Hendrix Yulis Setyawan
PY  - 2021
DA  - 2021/12/23
TI  - Comparative Study on the Extraction and Quantification of Polyphenols from Moringa oleifera Leaves Using Maceration with Pulsed Electric Field (PEF) as Pretreatment and Microwave-Assisted Extraction (MAE)
BT  - Proceedings of the International Conference on Innovation and Technology (ICIT 2021)
PB  - Atlantis Press
SP  - 142
EP  - 147
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.211221.019
DO  - 10.2991/aer.k.211221.019
ID  - 2021
ER  -