Quality and Quantity Test of DNA from Sperm in Water Immersion
- 10.2991/icpsuas-17.2018.41How to use a DOI?
- Criminal Case, Sperm, DNA Quality, DNA Quantity, STR-CODIS
Sperm is one of the body fluids that contain DNA and can be used as evidence or guidance to uncover various criminal cases. Evidence such as sperm can be found in various environments depend on the crime scenes such as being on a submerged cloth. The quality and quantity of DNA obtained from sperm is strongly influenced by the environment where the sperm are found. In this study, we tested the quality and quantity of DNA from sperm spots soaked in freshwater. The quality and quantity of DNA was measured using a spectrophotometer with a wavelength of 260 nm and 280 nm. The results showed an increase in the quality and quantity of DNA from sperm spots soaked on Day 1, 3 and 7. DNA ribbon also appeared on all samples when the DNA was amplified with STR primers at D21S11 and D13S317 locus.
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Abdul Hadi Furqoni AU - Wimbuh Tri Widodo PY - 2017/08 DA - 2017/08 TI - Quality and Quantity Test of DNA from Sperm in Water Immersion BT - Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017) PB - Atlantis Press SP - 192 EP - 194 SN - 2352-5398 UR - https://doi.org/10.2991/icpsuas-17.2018.41 DO - 10.2991/icpsuas-17.2018.41 ID - HadiFurqoni2017/08 ER -