Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017)

Quality and Quantity Test of DNA from Sperm in Water Immersion

Authors
Abdul Hadi Furqoni, Wimbuh Tri Widodo
Corresponding Author
Abdul Hadi Furqoni
Available Online August 2017.
DOI
https://doi.org/10.2991/icpsuas-17.2018.41How to use a DOI?
Keywords
Criminal Case, Sperm, DNA Quality, DNA Quantity, STR-CODIS
Abstract

Sperm is one of the body fluids that contain DNA and can be used as evidence or guidance to uncover various criminal cases. Evidence such as sperm can be found in various environments depend on the crime scenes such as being on a submerged cloth. The quality and quantity of DNA obtained from sperm is strongly influenced by the environment where the sperm are found. In this study, we tested the quality and quantity of DNA from sperm spots soaked in freshwater. The quality and quantity of DNA was measured using a spectrophotometer with a wavelength of 260 nm and 280 nm. The results showed an increase in the quality and quantity of DNA from sperm spots soaked on Day 1, 3 and 7. DNA ribbon also appeared on all samples when the DNA was amplified with STR primers at D21S11 and D13S317 locus.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Cite this article

TY  - CONF
AU  - Abdul Hadi Furqoni
AU  - Wimbuh Tri Widodo
PY  - 2017/08
DA  - 2017/08
TI  - Quality and Quantity Test of DNA from Sperm in Water Immersion
BT  - Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017)
PB  - Atlantis Press
SP  - 192
EP  - 194
SN  - 2352-5398
UR  - https://doi.org/10.2991/icpsuas-17.2018.41
DO  - https://doi.org/10.2991/icpsuas-17.2018.41
ID  - HadiFurqoni2017/08
ER  -