Proceedings of the 11th International Dentistry Scientific Meeting (IDSM 2017)

In Vitro Chitosan Hydrogel Based Tetracycline Cytotoxicity Test on Fibroblast Viability

Authors
Andrew Andrew, Irma Ervina, Harry Agusnar
Corresponding Author
Andrew Andrew
Available Online May 2018.
DOI
https://doi.org/10.2991/idsm-17.2018.36How to use a DOI?
Keywords
tetracycline, chitosan, viability, cytotoxic
Abstract
Tetracycline has been widely used as a periodontal support treatment since it has broad-spectrum bone tissue penetration and it can inhibit native collagen. Chi-tosan hydrogel-based tetracycline is known to have strong antibacterial effects, but the cytotoxic effects on fibroblast have yet to be studied. Objective: to obtain the in vitro cytotoxicity effect of chitosan hydrogel-based tetracycline on fibro-blasts. Material and Method Chitosan hydrogel-based tetracycline (0.5%, 0.7% and 1%) and chitosan hydrogel without tetracycline is made by dissolving chi-tosan powder in citric acid. Tetracycline powder is then added to the solution. 3T3 fibroblast cells were cultured in a well microplate containing RPMI-1640 media inside an incubator. The viability assay is conducted using the MTT-assay meth-od and repeated 5 times. The colour degradation is read with a microplate reader. The mean viability of fibroblasts applied with chitosan hydrogel-based tetracy-cline (0.5%, 0.7% and 1%) and chitosan hydrogel is 86.5%, 85.36%, 80.99% and 90.85%, respectively. The highest mean cell viability in the group of fibroblasts applied with chitosan hydrogel-based tetracycline is in the 0.5% group. This value is not significantly different than the value in the 0.7% group, but it is significantly different than the value in the 1% group. Conclusion Chitosan hydrogel-based tet-racycline shows a low cytotoxic effect on 3T3 fibroblast cells.
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Proceedings
11th International Dentistry Scientific Meeting (IDSM 2017)
Part of series
Advances in Health Sciences Research
Publication Date
May 2018
ISBN
978-94-6252-513-9
ISSN
2468-5739
DOI
https://doi.org/10.2991/idsm-17.2018.36How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Andrew Andrew
AU  - Irma Ervina
AU  - Harry Agusnar
PY  - 2018/05
DA  - 2018/05
TI  - In Vitro Chitosan Hydrogel Based Tetracycline Cytotoxicity Test on Fibroblast Viability
BT  - 11th International Dentistry Scientific Meeting (IDSM 2017)
PB  - Atlantis Press
SN  - 2468-5739
UR  - https://doi.org/10.2991/idsm-17.2018.36
DO  - https://doi.org/10.2991/idsm-17.2018.36
ID  - Andrew2018/05
ER  -