In Vitro Chitosan Hydrogel Based Tetracycline Cytotoxicity Test on Fibroblast Viability
- Andrew Andrew, Irma Ervina, Harry Agusnar
- Corresponding Author
- Andrew Andrew
Available Online May 2018.
- https://doi.org/10.2991/idsm-17.2018.36How to use a DOI?
- tetracycline, chitosan, viability, cytotoxic
- Tetracycline has been widely used as a periodontal support treatment since it has broad-spectrum bone tissue penetration and it can inhibit native collagen. Chi-tosan hydrogel-based tetracycline is known to have strong antibacterial effects, but the cytotoxic effects on fibroblast have yet to be studied. Objective: to obtain the in vitro cytotoxicity effect of chitosan hydrogel-based tetracycline on fibro-blasts. Material and Method Chitosan hydrogel-based tetracycline (0.5%, 0.7% and 1%) and chitosan hydrogel without tetracycline is made by dissolving chi-tosan powder in citric acid. Tetracycline powder is then added to the solution. 3T3 fibroblast cells were cultured in a well microplate containing RPMI-1640 media inside an incubator. The viability assay is conducted using the MTT-assay meth-od and repeated 5 times. The colour degradation is read with a microplate reader. The mean viability of fibroblasts applied with chitosan hydrogel-based tetracy-cline (0.5%, 0.7% and 1%) and chitosan hydrogel is 86.5%, 85.36%, 80.99% and 90.85%, respectively. The highest mean cell viability in the group of fibroblasts applied with chitosan hydrogel-based tetracycline is in the 0.5% group. This value is not significantly different than the value in the 0.7% group, but it is significantly different than the value in the 1% group. Conclusion Chitosan hydrogel-based tet-racycline shows a low cytotoxic effect on 3T3 fibroblast cells.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Andrew Andrew AU - Irma Ervina AU - Harry Agusnar PY - 2018/05 DA - 2018/05 TI - In Vitro Chitosan Hydrogel Based Tetracycline Cytotoxicity Test on Fibroblast Viability BT - 11th International Dentistry Scientific Meeting (IDSM 2017) PB - Atlantis Press SN - 2468-5739 UR - https://doi.org/10.2991/idsm-17.2018.36 DO - https://doi.org/10.2991/idsm-17.2018.36 ID - Andrew2018/05 ER -