Eukaryotic Expression and Purification Technology of Glucosidase in Pichia Pastoris
- https://doi.org/10.2991/efood.k.201227.001How to use a DOI?
- Pichia pastoris, glucosidase, eukaryotic expression, protein purification, p-NPG
Glucosidase is one of the most important hydrolases in the carbohydrate metabolism pathway in human body. It is noteworthy that glucosidase was raising high attention in numerous researches, attributed to its functional values and biological activities. The expression, secretion, extraction, purification, and quantitative analysis of glucosidase occupies an important position in the research of glucosidase. The Pichia pastoris expression system has been developed for 50 years. With its unique advantages, the expression system has successfully expressed hundreds of heterologous proteins. This article briefly describes the process of high-efficiency expression, extraction and purification of glucosidase in the P. pastoris expression system, focusing on the expression, separation and purification of glucosidase in P. pastoris, as well as the quantitative and activity analysis methods of glucosidase. Affinity chromatography is used for the separation and purification of crude enzyme solution, and UV–visible spectroscopy can be used for enzyme quantification and activity analysis.
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- This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - JOUR AU - Weijie Zhou AU - Dongsheng Yao AU - Weibin Bai PY - 2021 DA - 2021/01/08 TI - Eukaryotic Expression and Purification Technology of Glucosidase in Pichia Pastoris JO - eFood SP - 406 EP - 409 VL - 1 IS - 6 SN - 2666-3066 UR - https://doi.org/10.2991/efood.k.201227.001 DO - https://doi.org/10.2991/efood.k.201227.001 ID - Zhou2021 ER -