Down-regulation of MEF2D via HBc Nanoparticle Mediated siRNA Inhibits HepG2 Cell Proliferation in vitro

DOI

10.2991/icimm-15.2015.44How to use a DOI?

Keywords

MEF2D; Hepatoma Cells; Proliferation; Gene Therapy; HBc Particles

Abstract

Objective: Recombinant hepatitis B virus nucleocapsid protein maintains the ability to form particles self-assembly. We tried to use non-virus-like particles HBc package wrapping foreign gene to protect and transfer siRNA of MEF2D gene to target position, in order to make a contribution to analyze the relationship between the expression of MEF2D gene and the proliferation of hepatocellular cells. Methods: Firstly, prepared si-MEF2D/HBc nanoparticles and transfected it into hepatocellular cells. The knockdown of MEF2D gene effects on cell proliferation was detected by MTT assay, XTT cell viability assay and CKK-8 assays. Result: The proliferation of treated hepatocellular cells were decreased significantly compared with control cells. Pretreatment of HepG2 cells with si-MEF2D/HBc complex inhibited cell growth in a dose-dependent manner. Conclusion: HBc particles is one kind of promising biological nanomaterials which has presented itself as an appropriate and valuable material for other aspects of nanoparticle in future study.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jing Kong
AU  - Xiaoping Liu
AU  - Jun Chen
AU  - Fang Fang
PY  - 2015/07
DA  - 2015/07
TI  - Down-regulation of MEF2D via HBc Nanoparticle Mediated siRNA Inhibits HepG2 Cell Proliferation in vitro
BT  - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials
PB  - Atlantis Press
SP  - 230
EP  - 235
SN  - 2352-5401
UR  - https://doi.org/10.2991/icimm-15.2015.44
DO  - 10.2991/icimm-15.2015.44
ID  - Kong2015/07
ER  -