eFood

Volume 2, Issue 2, April 2021, Pages 92 - 99

Dendrobine Suppresses Lipopolysaccharide-induced Gut Inflammation in a Co-culture of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophages

Authors
Huifan Liu, Jiaxi Liang, Gengsheng Xiao, Lukai Ma*, Qin Wang*
College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China
*Corresponding authors. Email: m1991lk@163.com; zhongkai_wq@126.com
Corresponding Author
Lukai Ma
Received 12 January 2021, Accepted 9 April 2021, Available Online 15 April 2021.
DOI
https://doi.org/10.2991/efood.k.210409.001How to use a DOI?
Keywords
Dendrobine, inflammatory bowel diseases, tight junctions, NF-κB pathway
Abstract

We evaluated dendrobine for its potential effect on Inflammatory Bowel Disease (IBD) progression using the intestinal epithelial Caco-2/RAW264.7 macrophage co-culture cell model. The results showed that dendrobine maintained the tight junction (TJ) proteins in co-cultured Caco-2 cells, suggesting its protective effect on intestinal integrity. Moreover, the findings indicated that the dendrobine group downregulated levels of tumor necrosis factor-α (TNF-α), proinflammatory Interleukin (IL)-1β and IL-6, and inflammatory markers such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in co-cultured RAW264.7 cells. Additionally, dendrobine-mediated anti-inflammatory effects were observed based on the inhibition of LPS-induced proinflammatory mediators of nuclear factor kappa B (NF-κB)-related signaling. These results demonstrated the protective effect of dendrobine at effectively ameliorating intestinal barrier disruption and inhibiting activation of the NF-κB pathway in macrophages through the intestinal barrier.

Graphical Abstract

Copyright
© 2021 The Authors. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
eFood
Volume-Issue
2 - 2
Pages
92 - 99
Publication Date
2021/04/15
ISSN (Online)
2666-3066
DOI
https://doi.org/10.2991/efood.k.210409.001How to use a DOI?
Copyright
© 2021 The Authors. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Huifan Liu
AU  - Jiaxi Liang
AU  - Gengsheng Xiao
AU  - Lukai Ma
AU  - Qin Wang
PY  - 2021
DA  - 2021/04/15
TI  - Dendrobine Suppresses Lipopolysaccharide-induced Gut Inflammation in a Co-culture of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophages
JO  - eFood
SP  - 92
EP  - 99
VL  - 2
IS  - 2
SN  - 2666-3066
UR  - https://doi.org/10.2991/efood.k.210409.001
DO  - https://doi.org/10.2991/efood.k.210409.001
ID  - Liu2021
ER  -