Dendrobine Suppresses Lipopolysaccharide-induced Gut Inflammation in a Co-culture of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophages
- https://doi.org/10.2991/efood.k.210409.001How to use a DOI?
- Dendrobine, inflammatory bowel diseases, tight junctions, NF-κB pathway
We evaluated dendrobine for its potential effect on Inflammatory Bowel Disease (IBD) progression using the intestinal epithelial Caco-2/RAW264.7 macrophage co-culture cell model. The results showed that dendrobine maintained the tight junction (TJ) proteins in co-cultured Caco-2 cells, suggesting its protective effect on intestinal integrity. Moreover, the findings indicated that the dendrobine group downregulated levels of tumor necrosis factor-α (TNF-α), proinflammatory Interleukin (IL)-1β and IL-6, and inflammatory markers such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in co-cultured RAW264.7 cells. Additionally, dendrobine-mediated anti-inflammatory effects were observed based on the inhibition of LPS-induced proinflammatory mediators of nuclear factor kappa B (NF-κB)-related signaling. These results demonstrated the protective effect of dendrobine at effectively ameliorating intestinal barrier disruption and inhibiting activation of the NF-κB pathway in macrophages through the intestinal barrier.
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Cite this article
TY - JOUR AU - Huifan Liu AU - Jiaxi Liang AU - Gengsheng Xiao AU - Lukai Ma AU - Qin Wang PY - 2021 DA - 2021/04/15 TI - Dendrobine Suppresses Lipopolysaccharide-induced Gut Inflammation in a Co-culture of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophages JO - eFood SP - 92 EP - 99 VL - 2 IS - 2 SN - 2666-3066 UR - https://doi.org/10.2991/efood.k.210409.001 DO - https://doi.org/10.2991/efood.k.210409.001 ID - Liu2021 ER -