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The invention of CRISPR-Cas9 allowed the wanted genome to be targeted and edited. Clustered regularly interspaced short palindromic repeats (CRISPR) combined with Cas9 protein together can be diverted to a guided location in the genome, editing the DNA strand. This achievement improved the human body’s...

IETF has specified Mobile IPv4 and Mobile IPv6 in RFC3344 and RFC3775 respectively, but not yet discussed Mobile IPv4/v6 in any published RFC. This paper proposes a scheme to solve one of Mobile IPv4/v6 problems which Home Agent (HA) locates in IPv6 network, and Correspondent Node (CN) locates in IPv4...

Anthocyanin is an important production of horticulture plant secondary metabolism, which is tightly concerns with fruit quality. There are lots of factors regulate plant anthocyanin biosynthesis including UV, which is one of the most significant factors, however, the effect mechanism details of UV are...

Complex of cell-THPC-thiourea-ADP with lanthanide metal ions Dy3+ has been prepared. The thermal stability and smoke suspension of the samples are determined by TG, DTA and cone calorimetry. Experimental data show that for the complexes of cell-THPC-thiourea-ADP with the metal ions, thermal decomposition...

Flexible polyurethane foam has been treated with borax, expanded graphite (EG), EG/borax as flame retardant, respectively. The thermal degradation of samples has been studied by thermogravimetry (TG) and differential thermogravimetry (DTG) in air. From the resulting data, kinetic parameters for different...

Recent developments of EEDI (energy efficiency design index) which is an indicator of ship’s energy efficiency level imposed the cost effectiveness of sea-going vessels. Optimization of the ship’s total energy system is studied in this paper. This study is focus on optimum load sharing control, which...

In this study, an acid and alkali resistance cationic polyacrylamide flocculants (P(AM-MAPTAC)) was synthesized through copolymerization of acrylamide (AM) and methacrylamido propyl trimethyl ammonium chloride (MAPTAC) by innovative ultrasonic initiation technology. The structure and thermal decomposition...

Water eutrophication have become serious ecological problems. White-rot fungi have been demonstrated to be a feasible means of control, but the genotoxicity mechanisms involved have not been reported. In this study, Cryptomonas obovata FACHB-1301 was co-cultured with Phanerochaete chrysosporium under...

In this work, a sensitive E-DNA sensor based on avidin modified electrode and DNA-functionalized Au nanoparticle (DFNP) was developed. The DNA-Functionalized Au nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’ terminal and a thiol at the 5’ terminal, the other...

In the present study, we describe an electrochemical sensor for codeine detection by using the DNA aptamers against codeine. In the sensing protocol, a dually-labeled DNA Aptamer probe was designed to be labeled at one end with HS, and at its another end with an dabcyl as an electrochemical tag to produce...

Epoxy resins modified with low-viscosity hyperbranched epoxy were prepared. The tensile strength, flexural strength, impact strength, fracture toughness, glass transition temperature (Tg) of the cured samples were investigated. The results show that the addition of hyperbranched epoxy improves the overall...

New free radical-cationic hybrid photopolymer, consisting of acrylate-based and epoxy-based photosensitive resin for stereolithography was prepared. The influence of the initiator sort and amount on the curing degree of the photopolymer was investigated. Photopolymerization process, degradation temperature,...

In this study, the pyrolysis of Zichang˚Coal (ZC) was investigated by thermogravimetry (TG) using three˚Catalysts, and the yields of the products obtained from pyrolysis, as well as gas˚Composition, were investigated by gas˚Chromatography (GC) using a fixed-bed reactor. The˚Catalytic mechanism was discussed...

Nanocrystalline CeO2 powders were synthesized using stearic acid and cerium nitrate by sol-gel and self-propagating combustion coupled method. Influence of various molar ratio of stearic acid and cerium nitrate on the products grain and colour were discussed. Powder appearance, crystal phase, specific...

In light of urgent need of approval of ballast water management system against the new G8 guidelines, organism regrowth within treated ballast water should be assessed. However, it’s the first time for mandatory demands on the evaluation of organism regrowth in ballast water. For the existances of DNA...

What is the main cause of aging has been discussed over last decades. Accumulating evidences has indicated that genome instability including mitochondrial DNA in somatic cell played an important role in human aging process. Various sources of damage such as reactive oxygen species and UV radiation can...

This paper presents a system designed for automated nucleic acid extraction machine based on magnetic bead separation method. A hardware platform has been established which consists of motor controlling module, temperature controlling module and a computer; also, we designed the system software to achieve...

With the rapid development of China's economy and the gradual improvement of the socialist modernization, science and technology has been the development and application. While human research biotechnology, we also have a new understanding of animals genetic engineering. To some extent, promoting the...

The aim of this research is to automate an analysis of the EGFR gene as a whole, and especially an analysis of those exons with clinically identified microdeletion mutations which are recorded with non-mutated nucleotides in a long chains of a, c, t, g nucleotides, and “-“ (microdeletion) in the NCBI...

He 32 strains genetically stable and vigorous lactic acid bacteria isolated from fermented yak milk made by tibetans in Sichuan that were performered microscopic examination ,including 10 strains of Lactobacillus and 22 strains of Lactococcus after cultured, followed DNA extraction, PCR amplification....

Designed a DNA-based genetic algorithm under the universal architecture of organic computing, combined particle swarm optimization algorithm, introduced a crossover operation for the particle location, can interfere with the particles’ speed, make inert particles escape the local optimum points, enhanced...

TiO2-SiO2 film were prepared by sol-gel process. The prepared samples of these films have been characterized by FT-IR spectroscopy, TG-DTA, XRD and AFM. The transmittance of these films is tested by UV-VIS spectroscopy. The results show that the double doped glass antireflection film antireflection performance...

Natural silk fibers were used as the template to synthesis biomorphic Fe2O3 fibers. Silk fibers were first immersed into a Fe(NO3)3 solution and then sintered in air at high temperatures to produce the final Fe2O3 fibers. Their microstructures, phases, and synthesis process were analyzed. The results...

IETF has specified Mobile IPv4 and Mobile IPv6 in RFC3344 and RFC3775 respectively, but not yet discussed Mobile IPv4/v6 in any published RFC. This paper proposes a scheme to solve one of Mobile IPv4/v6 problems which Home Agent (HA) is located in IPv4 network, Correspondent Node (CN) is located in IPv6...

To develop the rapid method for detecting of Spring viremia of carp virus (SVCV) and Cyprinid herpesvirus 2(CyHV2), 2 pairs of specific primers for duplex reverse transcriptase polymerase chain reaction (RT-PCR) were designed according to the nucleotide sequence information of CyHV2 and SVCV published...

Research the pyrolysis kinetics of eucalyptus and the absorption band and characteristic peaks of volatile pruducts under the heating rate 10?•min-1, 30?•min-1, 60?•min-1respectively. The experimental results show that eucalyptus pyrolysis process can be divided into 3 stages: moisture volatilizes stage,...

In this work, a series of ternary rare earth complexes were synthesized with salicylidene glycine schiff base (Sal-GlyK) as anion ligand and 2,2'-bipyridine (bipy), l, 10-phenanthroline (phen) as neutral ligand. The antibacterial activities and interactions between calf thymus DNA (ct-DNA) in Tris-HCl...

Objective: A number of traditional Uyghur medicines have been recognized as potent antitumor agents and have been developed and analyzed in vivo. In the present study, the toxic, therapeutic and cytotoxic properties of the Uyghur medicine Abnormal Savda Munziq (ASMq) were investigated. In the presence...

A terpolymer resin (NSMA) was synthesized with styrene, maleic anhydride and N-phenyl maleimide. The structure and composition was characterized with FT-IR and 13C-NMR. The thermal resistance of NSMA was determined through integrated thermal analysis. The results indicated that, when the molar ratio...

we study the use of gatifloxacin with a novel tricyano-dihydrofuran fluorescent system. Gatifloxacin is a 8-methoxy-fluoroquinolone racemate which, in addition to having a broad spectrum of Gram-negative activity in addition to the activity of positive microorganisms, this product has antibacterial effect,...

DNA methylation is an important epigenetic modification mechanism, interacting with histone modification, changing the structure of chromatin and regulating gene expression. Meanwhile, there is a close relationship with cell proliferation, aging, cancer and other life phenomena. DNA methylation includes...

Identification and characterization of gene regulatory binding motifs is one of the fundamental tasks toward systematically understanding the molecular mechanisms of transcriptional regulation. Recently, the problem has been abstracted as the challenge planted (l,d)-motif problem. Previous studies have...

Purpose To establish a practical, fast and accurate identification method of tortoise shell, falsify amplified polymorphism DNA (RAPD) randomly and provide reliable basis for identification of tortoise shell. Method We use the Alkali cracking method to extract mitochondrial DNA from the tortoise shell...

As a resplendent jewel of China’s traditional intangible cultural heritage, paper-cutting art embodies the cultural DNA distilled over millennia and a distinctive aesthetic paradigm. The dual onslaught of modernisation and the digital revolution have plunged this ancient craft into a crisis of transmission...

The Mesozoic sequence framework of the eastern Yihezhuang salient in Jiyang depression, which was a residual basin, was studied in order to improve the success rate of locating hydrocarbon reservoirs. First, through seismic profiles, well logs and cores, complemented with outcrops, totally 5 sequence...

The pollution of textile dyeing wastewater is of highly concern for its notorious biotoxicity, especially the genotoxicity, which can cause DNA damage and chromosome aberration to aquatic flora and fauna. In this study, a lab-scale reactor system was constructed to study the change of genotoxicity during...

The covid-19 pandemic that occurred in the beginning of 2020 has affected all sectors, particularly in health and economic sectors. The Avian Influenza Virus (AIV), a zoonotic virus that have been reported endemic in Indonesia since 2003, might be affected by this condition. There is very limited information...

Based on results of biomass combustion and pyrolysis using TG-DSC analyzer, biomass thermodynamics characteristics were studied. Integrated heat model and differential heat model were used to determined biomass thermal behavior, and the former fitted the actual better; the heat capacities with temperature...

Quantum Dots are powerful imaging and tracking tools for a wide range of biomedical applications, especially in preparing molecular beacons, but its use is limited by the sensitivity of the electrostatic binding between streptavidin and QDs. In this investigation, we used EDC as a crosslinker and NHS...

There are various factors affecting drying shrinkage of cement-based material, including use quantity of cement and auxiliary cementitious material, water cement ratio, maintenance system and drying condition, and in addition, total quantity of coarse and fine aggregate, matching and category also affect...

As an excellent tear agent of the second generation, CS has been widely used in the police department of many countries. CS disperses the crowd depending on the aerosol in the air due to combustion and explosion. And at the same time, CS decomposes into a variety of products. With the use of TG/DSC-FITR...

The Ni-Co/HZSM-5 methane dehydroaromatization (DHA) catalyst was prepared using wet impregnation. The catalysts were characterized by XRD, TEM, TG-DSC, FT-IR and XPS techniques before and after reaction. The results show that the maximum conversion rate of methane DHA at 580 reached 39.8%. The carbon-silicon-oxygen...

For the problem of lower safety performance in literature [1] , an improved chaotic map gray image encryption algorithm based on information entropy has been proposed.The algorithm includes three portions: first,chaotic mapping generates random matrices to diffuse plaintext image matrix ; Second, DNA...

We designed and evaluated a kind of Agkistrodon DNA detection kit. Bioinformatics was used to investigate the characteristics of cytochrome b (cyt b ) gene in Agkistrodon. A series of primers were designed, and sequencing and blasting in conjunction with PCR technology were applied. Its’ indexes including...

Coccidiosis continues to be one of the most important issues in animal health and generates significant economic losses to the livestock industry in Indonesia, specifically due to Eimeria spp. Infections. Eimeria are specific to a particular host Eimeria and multi-species infections are more prevalent...

The addition of nano-SiO₂ to 20% silane coupling agent is carried out using anhydrous ethanol as the solvent in the presence of acetic acid. The styrene-butadiene-styrene (SBS) is added with 1% dicumyl peroxide crosslinking agent by adding 5% maleic anhydride grafted polyethylene to be modified on an...

Silver doped TiO2 catalyst TiO2-Ag was synthesized by microwave hydrothermal method when the optimal condition was selected with orthogonal and single factor tests. The photocatalytic activity of TiO2-Ag catalyst was further enhanced by the microwave and ultrasonic assisted with using methyl orange as...

DTA/SDTG and DSC techniques were used to study the thermal behaviors of citronellol- -D-glucopyranoside (CGLY) . The heating rate was 5, 7.5, 10, and 12.5 K min-1, the samples were heated from 30oC to 500oC. Methods of Friedman and Flynn-Wall-Ozawa were applied to calculate the parameters of thermal...

A new linear 10-undecenoic acid-based diol and a chain extenders with 12C dangling chain length were synthesized via thiol-ene coupling and esterification reaction. In order to investigate the effect of the 10-undecenoic acid-based diol chain extender, the mole percent of the 10-undecenoic acid-based...

nanopore; PNP equation; EOF; current signals