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In an attempt to isolate high-quality, total RNA from flower buds of sunflowers (Helianthus annuus). We have already improved the SDS method. After adding liquid nitrogen, we have to grind the material down. After that we add protein -DNA precipitation, wash it with the addition of different kinds of...

The selection step of plant breeding is a crucial stage in the process of developing new varieties which usually take a considerable long time in a conventional breeding. The development of molecular markers for selection has been carried out to reduce the time required. A Sequence Characterized Amplified...

The study was conducted within the individual genotyping of healthy and sick women in the Kabardino-Balkarian Republic to determine the risk-prone groups with increased susceptibility to hereditary form of breast cancer development. Blood samplings were collected and total DNA was taken from 300 people...

To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Pulsatilla chinesis (Bge.) Regel., we extracted the genome DNA from the leaves of Pulsatilla.dahurica (Fisch.) Spr., collected in Jilin Province, amplified the ITS and trnL-F region using...

The study aims to understand the interferon-gamma (IFN-γ) level and affecting factors in diabetes mellitus (DM) patients at Public Health Center (PHC). This cross-sectional study was conducted on 153 DM patients. The inclusion criteria were male or female patients over 18 years old diagnosed with DM...

The interaction towards a risk of breast cancer in the ethnically homogeneous group of the Chechens and the polymorphism of DNA repair genes was studied: XPC (rs2228000, rs2228001) XPA (rs 1800975) ERCC2 (rs13181; rs1799793). DNA samples taken from venous blood of 241 women diagnosed with breast cancer...

Wildlife conservation and enforcement strategies have been transformed drastically with the use of artificial intelligence, drones and DNA based forensic technology. To monitor wildlife habitats, prevent poaching and to strengthen evidentially base mechanism in wildlife crime prosecution these technologies...

The purpose to know the genetic variability of orchid variants Spathoglottis plicata based on molecular marker Random Amplified Polymorphic DNA (RAPD). DNA isolation carried out using TIANGEN Plant Genomic DNA kit. DNA sample was amplified using PCR-RAPD technique. Genetic variability were analyzed using...

Cardiovascular diseases are the main cause of mortality and morbidity in Western countries, but the underlying mechanisms are still poorly understood. Genetic polymorphisms, once thought to represent a major determinant of cardiovascular risk, individually and collectively, only explain a tiny fraction...

Abstract. Objective: To study the protection role of the quercetin on the ischemia-reperfusion injury liver rat. Methods: The gastric gavage quercetin and as a positive control of vitamin C, 30 m in the liver door open after ligation of vascular cause liver ischemia-reperfusion injury, respectively,...

Background: SNPs on chromosome 9p21.3 risk locus have been associated with cardiovascular diseases. We have established a direct mechanistic link between 9p21 noncoding risk interval and susceptibility to aneurysm in a mouse model with a targeted deletion of the 9p21 noncoding cardiovascular disease...

Malaria is caused by a parasitic infection of the genus Plasmodium. Plasmodium falciparum is the cause of almost all deaths caused by malaria. Malaria problem has been exacerbated in recent years by the development and rapid spread of P. falciparum resistance to antimalarial drugs. Resistance to antimalarial...

The growth hormone receptor (GHR) gene plays an important role in regulating growth as a receptor for the growth hormone (GH) gene. This study aims to analyze the variation of the GHR|Eco72I gene and its association with body size in the Maron Chicken elders. Ninety DNA samples were extracted from the...

Vascular stiffening and calcification are hallmarks of ageing and these pathologies are accelerated in patients with diabetes and renal failure. Emerging evidence has defined a role for nuclear lamina defects and the DNA damage response in driving vascular calcification. The mechanisms responsible are...

Cellular damage induced by free-radicals like reactive oxygen species(ROS) has been implicated in several diseases. The ROS radicals like alkoxy radical(RO-) and peroxy radical (ROO-) are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events...

The aim of this research is to determine the level of genetic diversity of in vitro cultur Spathoglottis plicata variants derived irradiated (mutants) and without irradiation (wild type) seeds. This research is an observation study to detect of genetic diversity of plant variants based on ISSR molecular...

Systemic Lupus Erythematosus (SLE) is a chronic multisystem autoimmune disorder characterized by the development of autoantibodies and immune complexes in association with a wide variety of clinical manifestations and tissue damage including oral mucosa. Oral lesions were non-spesific in SLE, may be...

The Tadulako site is one of the sites characterized by megalithic culture in the Behoa Valley, Poso Regency, Central Sulawesi Province. The presence of megalithic cultural remains at this site was first recognized by foreign researchers around the 19th century AD. Research in this area is still rarely...

Abstract. To observe hypoglycemic and hypolipidemic effects of Melastoma dodecandrum ethanol-extract on type 2 diabetic rats. Except twelve SPF SD rats were as the control group, the rest eighty type 2 diabetic rats were induced by feeding with high-fat and high-sugar diet and intraperitoneal injection...

The Yakushevich model provides a very simple pictures of DNA torsion dynamics, yet yields remarkably correct predictions on certain physical characteristics of the dynamics. In the standard Yakushevich model, the interaction between bases of a pair is modelled by a harmonic potential, which becomes anharmonic...

The development of S. plicata orchid variants is important to increase their genetic diversity. This study aims to determine the genetic diversity of S. plicata orchid variants based on RAPD analysis. The research sample was 15 plant variants consisting of wild type and mutant orchid variants. Molecular...

Pore structures and aluminum foam densities are strongly influenced by the particle size of TiH2 powders. In this paper, TG curves of the the original powders and the 4 kinds of powders which were separaed by the original powders were researched. The foaming process and pore structures using selected...

This study aims to evaluate gut microbiota colonization in the first two years of a child’s life and explore the potential of the gut microbiota for the development of in vivo diagnostics (IVD) of degenerative diseases by reconstructing the dynamics of early colonization of gut microbiota at a genomic...

To investigate the correlation among serum lipids and their ratios and cystatin C (CysC) in patients with hyperthyroidism. authors respectively chose fifty patients of hyperthyroidism, subclinical hyperthyroidism and the control group. TC, TG and LDL-C in the hyperthyroidism and subclinical hyperthyroidism...

Adenovirus is a DNA virus which was discovered in 1950s from military recruit with atypical pneumonia. The majority of the higher-level number for serotypes has been insulated from immunocompromised persons. While the majority of infections linked to adenovirus are self-limiting, those who occur in immunocompromised...

Synedrella nodiflora (L.) Gaertn and Eleutheranthera ruderalis (Swartz) Sch.-Bip. are two Asteraceae species showing very similar morphological appearances. Both are broad-leaf weed species in some crops throughout many tropical areas. Nevertheless, the individual species can be potentially utilized...

It is to offer methodological guideline for designers in product form design, to better meet the demands of users. Based on the visual perception dynamics theory, DNA molecular sequencer is selected as a design example to analyze the functional mechanism of visual perception dynamics. It confirms the...

This paper describes a novel approach utilizing nano-graphite as an effective fluorescent sensing platform for highly sensitive and selective detection of Ag+ in aqueous solution for the first time. Nano-graphite can effectively quench the fluorescence of dye-labeled cytosine-rich single-stranded DNA...

About 85% of Indonesia’s population is Moslem. Every Moslem is ordered to eat halal and good food. PCR is the most widely used molecular biology technique for authentication tests, through target DNA analysis, including the detection of pork-derived DNA in food products. DNA isolation is an important...

Background: A neurodegenerative disorder in the elderly individuals. It is a type of dementia that affects memory subsequently followed by executive dysfunction, confusion, agitation, and behavioral disturbances. Three causative genes have been associated with autosomal dominant familial AD (APP, PSEN1,...

Background: Previous studies have demonstrated poor metabolic and blood pressure control in the diabetic population in Trinidad. The aim of this study is to compare baseline and follow-up metabolic parameters and blood pressures taken within a 16-month period to ascertain if there have been improvements. Method:...

CD30 is expressed in Hodgkin lymphoma (HL). To date, research efforts have focused on understanding its contribution to lymphomagenesis through anti-apoptotic mechanisms, and its effect on cell survival. It has been suggested that Epstein-Barr virus (EBV) plays a role in the pathogenesis of positive...

Plasmodium is causative agent of malaria in human through the intermediary of female mosquitoes Anopheles spp. Two of the most important human malaria parasites, P. falciparum and P. vivax, are derived from the complete transmission event of non-human primate malaria species to human. Late data shows...

The aim of this study was to assess the quality of Gaga chicken spermatozoa during freezing using lactated ringer diluent added with skim milk. This study used a completely randomized design (CRD) with 4 treatment levels (control, skim milk 5%, 10% and 15%) and 10 replicates. The research stages were...

Cardiovascular diseases are the main cause of mortality and morbidity in Western countries, but the underlying mechanisms are still poorly understood. Genetic polymorphisms, once thought to represent a major determinant of cardiovascular risk, individually and collectively, only explain a tiny fraction...

This article represents the results of studies of the microorganism resistance to antimicrobial agents detected by PCR in the milk of cows and goats. In 94.1% of cow milk and in 80.7% of goat milk samples specific DNA regions of microorganisms were detected: Staphylococcus spp., E. coli, S. aureus, S....

In the present work, hydroxyapatite (HAp) was sustainably extracted from fish-bone biowaste and converted into a functional ceramic material through appropriate thermal treatment. The thermal behavior of the biogenic precursor was investigated using thermogravimetric and differential thermal analysis...

“Ensuring that every guilty person is subject to criminal punishment and protecting innocent people from criminal prosecution” is the ultimate goal of formal justice. In recent years, cases of unjust, false and wrong have occurred frequently in China, exposing various problems in Chinese judicial system....

Sandelwood horse is one of local horses in Indonesia that has become known for its use in horse racing competition. Many genes are known to have an effect on performances traits, but the role of one gene, Myostatin (MSTN) has not been explored yet. This gene is responsible for embryonic and adult muscle...

In the experiment, the grasshopper in Siberia is taken as the research objec. The comparison of the genomic DNA of dried and soaked in alcohol samples are extracted by the method of saturated NaCl, CTAB and SDS. Experimental results show that SDS method brings the total DNA, saturated Nacl law and CTAB...

To develop a rapid method for the detection RHDV2, 4 specific primers and 12 overlapping PCR primers were designed to amplify the conserved RHDV2 specific DNA fragment according to the genome sequences of RHDV and RHDV2 published in GenBank. Based on the synthesis of a conserved part of the RHDV2 sequence...

Moringa oleifera, which is endemic of Pulau Poteran, Madura, is one of a genetic variation of Moringa. It is native Indonesian germplasm, which must be preserved to maintain the genetic biodiversity of Moringa. Known locally as kelor, the plant has four different color of petiole: red, white, purple,...

Locally known as top predator fishes, peacock bass or cichlids are invasive in Malaysia’s freshwater ecosystem. Due to the nature of these fish, which involves hiding beneath the water’s surface, detection probability for these species are often poor, especially when utilizing the traditional capture-survey...

Background: Schizophrenia is a complex disorder involving multiple genes with mild to moderate effects and non-genetic risk factors such as environmental and psychological influences. A significant reduction in interleukin-2 -330 T/G production by peripheral lymphocytes is an immunological finding replicated...

The influence of hygrothermal environment on compressive property of woven carbon fiber epoxy resin composite was investigated via tensile and compress tests under different hygrothermal environment. Moisture absorption, tensile and compressive properties, failure modes, FTIR and dynamic mechanical property...

Investigation of the prevalence and risk factors of human papillomavirus (HPV) infection is the basis for developing prophylactic strategies against cervical cancer, especially for young women. This study aimed to assess the prevalence and risk factors of HPV infection among a cohort of sexually active...

Asthma is a chronic inflammatory disease characterized by airway obstruction and hyper-responsiveness. It is a complex disorder influenced by genetic and en-vironmental factors, which contribute to the clinical manifestations of asthma. Asthma may affect the lower respiratory tract, causing epithelial...

The adsorption of Cs+ by a novel extractant-impregnated resin (EIR), was studied using batch adsorption experiments. The new EIR was prepared by impregnating 4-tert-butyl-2-( -methylbenzyl) phenol (t-BAMBP) onto AB-8 macroporous resin beads. Thermogravimetric analysis (TG) and the Fourier Transform Infrared...

Gene expression is the process of reading from DNA into a functional product. Regulation of gene expression through complex mechanisms. HIF1-1α is one of the regulatory genes that regulates the return of homeostasis when there is a lack of oxygen (hypoxia). Hypoxia is often experienced by athletes during...

The application of nanotechnology is significant in that it is exploited substantially, such as employing CNTs for targeted delivery of anti-cancer drugs. Through molecular dynamics (MD) simulations with Materials Studio Software, the anti-cancerous carbon nanotube drug delivery system for supporting...