Proceedings of the 2016 International Conference on Biological Sciences and Technology

Session: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues

21 articles
Proceedings Article

Clinical Practicability of Extending the Length of Gastrointestinal Decompression Cathetering

Lei Sun, Xin-Quan Gu, An-Na Wang, Fan-Ping Meng
Objective: We intended to investigate the appropriate length of the stomach tube in the treatment of gastrointestinal decompression. Material and Methods: The patients in treatment of gastrointestinal decompression cured by our department from January 2013 to May 2015 whom were divided into 2 groups...
Proceedings Article

Quantitative Structure-Toxicity Relationship for Predicting Acute Toxicity of Phenols

Zhi-Xiang Zhou, Meng-Nan Qin, Yang-Hua Liu, Xiao-Long Zhang, Han-Dong Li
Phenols represent one of the most important classes of environmental chemicals. Most of them may cause serious public health and environmental problems. The present work is to develop an effective QSTR model for acute toxicity, a toxicological endpoint of Phenols. We calculated various descriptors and...
Proceedings Article

Research on Morphology of Human Red Blood Cells (RBCs) in Different Gravity Fields

Lin Li, Shuang-Yin Gao
By testing fresh human red blood cells (RBCs), the morphology of human RBCs in different gravity fields was observed by SK2109P microscope. And the death rate of RBCs in different gravity fields were counted Results show that: RBCs can endure a certain gravity field. RBCs barely died under gravity fields...
Proceedings Article

To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus

Ying-Ying Liu, Jing Wang
Acridine orange (AO) and ethidium bromide (EB) are two fluorescent dyes for cell nuclei staining. The nucleus images by these two staining can make it easy to differentiate white blood cell (WBC) from red blood cell (RBC). We applied this principle to the white blood cell count by hemocytometer and this...
Proceedings Article

Transurethral Flexible Ureteroscopic Holmium Laser Lithotripsy for Treatment of Kidney and Upper Ureteral Calculi

Fan-Ping Meng, An-Na Wang, Ji Gao
This paper aims at studying the efficacy and safety of flexible ureteroscopic holmium laser lithotripsy for treatment of kidney and upper ureteral calculi. Methods. The duration of operation, postoperative complication, postoperative calculus clearance rate of this surgery were analyzed based on 58 patients...
Proceedings Article

Investigation of Listeria Phage Endolysin Cell-wall Binding Domain (CBD) Surface Display in Escherichia coli

Shan-Na Liu, Timo M. Takala, Justus Reunanen, Ossian Saris, Per E. J. Saris
Cell surface display of target proteins has been widely used in biotechnology and industry. In this study, cell-wall binding domain (CBD) from Listeria phage endolysin A500 was fused with anchoring domains of YadA and OmpA, respectively, in Escherichia coli, aiming at binding of E. coli cells to Listeria...
Proceedings Article

Effect of Carnosine on Physico-Chemical and Antioxidant Activity Properties of FSG-CaCO3 Composite Films

Li-Hui Wang, Le Zhang, An-Jun Liu
For the sake of developing fish skin gelatin and CaCO3 composite films with antioxidant activity, different concentration of carnosine was added to it. In addition, the mechanical properties, optical properties, surface topography, anti-microbial activity, antioxidant activity and preservation effect...
Proceedings Article

Gene Prediction Based On a Generalized Hidden Markov Model and Some Statistical Models of Related States: a Review

Rui Guo, Jian Zhang, Ke Yan, Tian-Qi Wang
In recent years, the methods with a generalized hidden Markov model have gained significant application and development in gene prediction, which is predicting the location and structure of genes in genomic sequences, and produced an army of remarkable programs, such as Genie, GENSCAN, AUGUSTUS, etc....
Proceedings Article

The Separation and Purification of Se-enriched Mycelium Polysaccharides of Catathelasma Ventricosum and Their Anti-hyperglycemic and Antioxidant Activity

Yun-Tao Liu, Qian-Qian Tang, Yu-Xian You, Si-Qi Zeng, Yi-Wen Li, Di Chen, Ai-Ping Liu, Chao-Hui Feng, Zhao Su, Cheng Li, Dai-Wen Chen
Selenium (Se) is an essential nutrient for humans as it plays an important role in anti-hyperglycemic and antioxidant activities. The objective of this work was to evaluate anti-hyperglycemic and antioxidant activities of Se-enriched mycelium polysaccharides. The Se-enriched mycelium polysaccharides...
Proceedings Article

Differences between Aureobasidium Pullulans CGMCC No.3337 and Aureobasidium Pullulans TKPM10017 on Synthesis of Poly (β-L-malic-acid) Metabolism

Hai-Song Yin, Yan-Hui Bian, Wei-Hua Tang, Xu-Jia Fan, Zheng Li, Chang-Sheng Qiao
Poly (β-L-malic-acid) (PMLA) is a sort of polyester and absolute biodegradable material. PMLA is a new type of nontoxic macromolecule material and with favorable biodegradation and biocompatibility. PMLA has extensive applications in the areas of pharmacy and medical industry. Application foreground...
Proceedings Article

Preparation of Diuridine and Dicytidine Pentaphosphates

Yue-Hai You, Shan-Shan Gong, Qi Sun
Two symmetrical dinucleoside pentaphosphates, Up5U and Cp5C, were synthesized via the activation of the P (V)–N bond. The key nucleoside phosphoropiperidates intermediate were obtained by a redox condensation method. The reaction of the phosphoropiperidate with uridine and cytidine tetraphosphate in...
Proceedings Article

Immunoassays of Methionine Adenosyltransferase Activity, S-adenosylmethione and Their Applications

Xiu-Juan Hao, Hui-Jun Li, Min Zhou
To understand how S-adenosylmethione (SAM) fluctuates in normal and cancerous liver cells, we developed novel methods for measuring methionine adenosyltransferase (MAT) activity and SAM. This method integrated MAT-catalyzed reaction and immunoassay that specifically and quantifies product SAM. IHC, IF...
Proceedings Article

Coagulation Factor XII –A Key Pro-Inflammatory and Pro-Coagulant Protein

Shan-Shan Wang, Jin-Wen Ge, Shao-Wu Cheng
Coagulation factor XII (FXII) is a multidomain serine protease that is the starter of intrinsic coagulation pathway. FXII deficiency and clinical hemostasis are not associated with bleeding, so investigators have not considered FXII important in physiology for a long time. In seeking explanation for...
Proceedings Article

SD Rat Liver Polydatin Concentration Control Based On Sliding Mode with High-gain Observer

Jian-Yang Lin, Yu-Shuai Bu, Jin-Rui Gao
In this paper, we use the sliding mode control based on high gain observer to control the concentration of Polydatin in the liver of SD rates. Through data processing and with the help of the nlinfit function in MATLAB, we take a fitting analysis by using the Polydatin distribution data in SD rats’ liver...
Proceedings Article

The Research on the Mechanism of Antioxidative and Growth-Promoting Effects of Polyphenols from the Involucres of Castanea Mollissima Blume on IEC-6 Cells

Y. Xiong, X. Zhao, S. Dong, K.J. Guo
The aim of the paper was to study the effects of Polyphenolic extract from involucres of Castanea mollissima Blume (PICB) on IEC-6 cell proliferation and growth and antioxidant status. The IEC-6 heat stress model was established and IEC-6 cells of control group and heat stress group were treated with...
Proceedings Article

Preparation and Characterization of Antimicrobial Activity Bacterial Cellulose Membrane

Wei-Hua Tang, Shi-Ru Jia, Yuan-Yuan Jia
Bacterial cellulose (BC) shows good potentiality as active packaging material. However, bacterial cellulose itself has no antimicrobial activity to prevent microbial contamination. To achieve antimicrobial activity, -poly lysine ( -PL) was incorporated into BC by immersing BC in -PL solution. Investigations...
Proceedings Article

Analysis on the Performance of the Balb/c 3T3 Two-stage Assay in a Miniature Culture Area and in a Perfusion Culture Model

Vania H. M. Teofilo, Jin-Tao Li, Xin-Hui Liu, Ru-Gang Zhong, Si Ye, Zheng-Bo Zhao, Yi Zeng
The Balb/c 3T3 cell transformation assay (CTA) is presently undergoing extensive pre-validation testing but its potential for routine chemical screening remains to be fully exploited due it being more costly and more time consuming than other CTAs. To downscale the assay 24-well plates were used to score...
Proceedings Article

Mitochondrial Impairment Mechanism in D-galactose-induced Senescence in Experimental Fibroblast Cell Model

Wei Ye, Jian-Ming Cao, Xiao-Jun Tang, Chu Liu, Dong-Jie Hao, Jing Jin, Jian-Xin Lu
To establish an effective model for cellular senescence, human embryo lung fibroblast cells (MRC-5) were cultured in D-galactose (D-Gal) medium respectively, as control, same concentration of D-glucose (D-Glu) was used. Decrease in cell proliferation, increase in senescence associated -galactosidase...
Proceedings Article

The Research of Small Molecule Soybean Polypeptide Precipitated by TCA

Jian Xiong, Yu-Ke Xiang, Jun Ye, Yu-Hao Zhang
Trichloroacetic acid (tca) makes macromolecular peptides expose more hydrophobic groups and have precipitation effect. It’s widely used in the desalination and concentration of protein and peptide. This experiment use the alkaline protease enzymolysis the soybean protein isolated and makes the products...
Proceedings Article

Parameters Identification of Short Hanger Based on Adaptive Genetic Algorithm

Pei Yuan
The hanger tension is crucial in arch bridges, and the fundamental frequency of short hangers is significantly affected by the flexural rigidity. In order to measure the hanger tension considering the flexural rigidity, the Additional Mass Method (AMM) is applied. The tension and flexural rigidity can...
Proceedings Article

A Rate-limited Step Guided by Different Regulation of Chromatin Modifiers Should be Undergone after Initiation Phase in Cellular Reprogramming

Rong Hu, Xian-Hua Dai, Zhi-Ming Dai, Qian Xiang
At early stage and late stage of cellular reprogramming the transgenes arouse two waves of transcription and epigenetic abruptly, while it is relatively quiet in intermediate stage. In this paper we analyzed the gene expression and chromatin modification data at different time point of mouse embryonic...